Ketone body synthesis from leucine by adipose tissue from different sites in the rat

Archives of Biochemistry and Biophysics
Y Y Yeh

Abstract

Leucine is catabolized to ketone bodies in adipose tissue, but the contribution of this output to overall ketone metabolism is not known. The intent of the present study was to determine the capacity of different adipose tissues to synthesize ketone bodies from leucine. The amino acid was readily converted into acetoacetate in epididymal, perirenal, and omental fat tissues. In rats fed ad libitum, the rate of acetoacetate synthesis in omental fat (about 2 mumol g tissue-1h-1) was at least 8 times higher than in epididymal or perirenal fat. In omental fat, the rates of acetoacetate formation from alpha-ketoisocaproic acid were 47-55% lower than from leucine at all concentrations examined. There was no significant synthesis of beta-hydroxybutyrate from leucine or alpha-ketoisocaproic acid. After oxidative decarboxylation, a greater proportion (about three-fourths) of leucine in omental fat was metabolized to acetoacetate than to CO2 production through the Krebs cycle. Although addition of glucose, pyruvate, or carnitine did not affect the production of acetoacetate, fasting for 24 h stimulated acetoacetate synthesis from leucine and alpha-ketoisocaproic acid in omental fat. The high rate of leucine conversion to acetoacetate in o...Continue Reading

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Citations

Jul 1, 1993·Journal of Biochemical and Biophysical Methods·J A Fernández-LópezM Alemany
Oct 8, 2016·BMC Cancer·Laís Rosa VianaMaria Cristina Cintra Gomes-Marcondes
Jun 25, 2017·American Journal of Physiology. Heart and Circulatory Physiology·Edith RenguetLuc Bertrand
Sep 21, 1987·Life Sciences·P Hahn, M Taller

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