Abstract
An Affymetrix mouse genome array and differential in-gel electrophoresis (DIGE) techniques were used to investigate the pharmacological mechanisms of a mixture of herbs, designated CTCM, a compound of traditional Chinese medicine, for the treatment of increased permeability in mouse intestinal microvascular endothelial cells (MIMECs) induced by the Shiga-like toxin type II variant (SLT-IIv). MIMECs were challenged with 10microg/ml SLT-IIv for 12h and then treated with CTCM at a concentration of 200microg/ml for 12h. Total RNA and proteins from each treatment group were extracted from cultured MIMECs for analysis by the Affymetrix GeneChip Mouse Genome 430 2.0 microarray and DIGE. The results obtained demonstrated that there were one genes downregulated and one genes upregulated, one protein downregulated and four proteins upregulated in the SLT-IIv group compared to the control group. In the CTCM group, four genes were upregulated, three genes were downregulated, a single protein was downregulated and a single protein was upregulated when compared to the control group. When the CTCM-treated group was compared to the SLT-IIv group, expression of one gene was found to be increased, and all other genes were decreased, with five pr...Continue Reading
References
Jun 1, 1992·Veterinary Microbiology·H ImberechtsP Lintermans
Jan 1, 1990·General and Comparative Endocrinology·J M DangerH Vaudry
Feb 1, 1985·Journal of Molecular and Cellular Cardiology·R KandolfP H Hofschneider
Jan 7, 1998·Electrophoresis·M UnlüJ S Minden
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Jan 23, 1999·Nature Genetics·C Debouck, P N Goodfellow
May 16, 2000·The Journal of Biological Chemistry·A NandiM H Siegelman
Aug 19, 2000·Circulation Research·J S Alexander
Dec 29, 2000·Seminars in Thrombosis and Hemostasis·A BierhausP P Nawroth
Apr 6, 2001·Virology·S M AkulaB Chandran
Apr 20, 2001·The International Journal of Biochemistry & Cell Biology·F BussolinoB Ensoli
Dec 1, 2001·The Journal of Biological Chemistry·Hua Poo SuKodimangalam S Ravichandran
Dec 20, 2002·Nature·Carl Nathan
Aug 23, 2003·Infection and Immunity·Markus EsslerMartin Aepfelbacher
Oct 7, 2003·FEBS Letters·Amy M McGoughKaren D Simonetti
Jun 9, 2004·Trends in Cell Biology·Cédric Polesello, François Payre
Jun 10, 2004·Proteomics·Natasha A KarpKathryn S Lilley
May 19, 2005·Analytical and Bioanalytical Chemistry·Rita MarougaEdward Hawkins
Nov 1, 2005·Journal of Ethnopharmacology·Huiqin DuanXiang Mu
Nov 9, 2005·Cell Biology International·Yun WangTian Yang
May 25, 2006·Journal of Molecular Endocrinology·R BuettnerL C Bollheimer
Oct 20, 2007·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Honglei LiCunshuan Xu
Dec 31, 2014·Cellular Reprogramming·Qing HuanZi-Jiang Chen
Feb 24, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ling-Fang WuLan-Zhen Zhang