Key role of hydrogen peroxide in antimicrobial activity of spring, Honeydew maquis and chestnut grove Corsican honeys on Pseudomonas aeruginosa DNA

Letters in Applied Microbiology
J-P PoliV Lorenzi

Abstract

In honeys, several molecules have been known for their antibacterial or wound healing properties. Corsican honeys just began to be tested for their antimicrobial activity with promising results on Pseudomonas aeruginosa. So, identification of active molecules and their mode of action was determined. Hydrogen peroxide concentrations were evaluated and, in parallel, the minimal inhibitory concentrations (MIC) values were performed with and without catalase. More, the quantity of phenolic compounds and ORAC assay were measured. Observation of antibacterial action was done using scanning electron microscopy (SEM) followed by plasmidic DNA extraction. MIC values of chestnut grove and honeydew maquis honeys vary between 7 and 8%, showing a strong antimicrobial capacity, associated with a plasmidic DNA degradation. When catalase is added, MIC values significatively increase (25%) without damaging DNA, proving the importance of H2 O2 . This hypothesis is confirmed by SEM micrographies which did not show any morphological damages but a depletion in bacterial population. Although, such low concentrations of H2 O2 (between 23 μmol l-1 and 54 μmol l-1 ) cannot explain antimicrobial activity and might be correlated with phenolic compounds c...Continue Reading

References

Mar 20, 1998·Infection·H A Wahdan
May 14, 1999·Current Opinion in Microbiology·G Storz, J A Imlay
Oct 9, 1999·Clinical Microbiology Reviews·M M Cowan
Sep 13, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jamshid L ManzooriMaghsood Orooji
May 3, 2007·Canadian Journal of Microbiology·Katrina Brudzynski
Mar 17, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Paulus H S KwakmanSebastian A J Zaat
Oct 23, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jin Dai, Russell J Mumper
Mar 17, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Igor JerkovićMladenka Malenica Staver
Sep 6, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hasan A AlzahraniBalkees A Bakhotmah
Jun 14, 2013·BMC Complementary and Alternative Medicine·Mohd Izwan ZainolMohd Yasim Mohd Yusof
Jan 1, 2014·International Journal of Bacteriology·Michelle E M Campeau, Robin Patel

❮ Previous
Next ❯

Citations

Mar 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Patricia Combarros-FuertesM Eugenia Tornadijo
Apr 24, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcela BucekovaJuraj Majtan
Mar 18, 2020·Journal of Food Science and Technology·Michał GoślińskiLucyna Kłębukowska
Nov 8, 2020·Antibiotics·Patricia Combarros-FuertesLeticia M Estevinho
Jun 22, 2021·Access Microbiology·Annabel GuttentagDee Carter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.