Key role of piRNAs in telomeric chromatin maintenance and telomere nuclear positioning in Drosophila germline

Epigenetics & Chromatin
Elizaveta RadionAlla Kalmykova

Abstract

Telomeric small RNAs related to PIWI-interacting RNAs (piRNAs) have been described in various eukaryotes; however, their role in germline-specific telomere function remains poorly understood. Using a Drosophila model, we performed an in-depth study of the biogenesis of telomeric piRNAs and their function in telomere homeostasis in the germline. To fully characterize telomeric piRNA clusters, we integrated the data obtained from analysis of endogenous telomeric repeats, as well as transgenes inserted into different telomeric and subtelomeric regions. The small RNA-seq data from strains carrying telomeric transgenes demonstrated that all transgenes belong to a class of dual-strand piRNA clusters; however, their capacity to produce piRNAs varies significantly. Rhino, a paralog of heterochromatic protein 1 (HP1) expressed exclusively in the germline, is associated with all telomeric transgenes, but its enrichment correlates with the abundance of transgenic piRNAs. It is likely that this heterogeneity is determined by the sequence peculiarities of telomeric retrotransposons. In contrast to the heterochromatic non-telomeric germline piRNA clusters, piRNA loss leads to a dramatic decrease in HP1, Rhino, and trimethylated histone H3 ly...Continue Reading

References

Dec 22, 1998·Molecular and Cellular Biology·O N DanilevskayaM L Pardue
Jan 9, 1999·Chromosoma·L FantiS Pimpinelli
Feb 12, 2002·Nature Reviews. Genetics·Heather C Mefford, Barbara J Trask
Apr 10, 2002·Molecular and Cellular Biology·Mikhail SavitskyPavel Georgiev
Jan 3, 2003·Nature Cell Biology·Giovanni CenciMaurizio Gatti
Nov 18, 2003·Annual Review of Genetics·Mary-Lou Pardue, P G DeBaryshe
May 28, 2004·Molecular Biology and Evolution·José P AbadAlfredo Villasante
Jun 4, 2004·Molecular Biology and Evolution·José P AbadAlfredo Villasante
Sep 7, 2005·Genetics·Harald BiessmannJames M Mason
Feb 3, 2006·Genes & Development·Mikhail SavitskyVladimir Gvozdev
Sep 12, 2006·Genome Research·Janet A GeorgeMary-Lou Pardue
Feb 10, 2007·Cell·Tomoyasu SugiyamaShiv I S Grewal
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Jun 16, 2010·PLoS Genetics·Jan VrbskyKarel Riha
Dec 24, 2010·PLoS Genetics·Jaspreet S KhuranaWilliam E Theurkauf
Jul 19, 2011·Nucleic Acids Research·Sergey ShpizAlla Kalmykova
Sep 13, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·J DufourtC Vaury
Nov 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mikhail S KlenovVladimir A Gvozdev
Nov 19, 2011·RNA·Felix MuerdterAlexei A Aravin
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sidney H Wang, Sarah C R Elgin
Feb 18, 2012·Annual Review of Physiology·Cathleen M Lake, R Scott Hawley
Aug 28, 2012·Nature·Augustin de VanssayStéphane Ronsseray
Feb 9, 2013·Genes & Development·Nikolay V RozhkovGregory J Hannon
Feb 9, 2013·Genes & Development·Adrien Le ThomasKatalin Fejes Tóth
May 24, 2013·Genome Biology and Evolution·Miguel GaravísAlfredo Villasante
Jul 31, 2013·Genetics·Natalia WesolowskaYikang S Rong

❮ Previous
Next ❯

Citations

Nov 18, 2018·Nature Reviews. Genetics·Deniz M OzataPhillip D Zamore
Jun 21, 2019·Biochemistry. Biokhimii︠a︡·M Yu Kordyukova, A I Kalmykova
Jan 11, 2020·Frontiers in Cell and Developmental Biology·Negin KhosravianiKarim Mekhail
Dec 23, 2020·ELife·Claudia Castillo-González, Dorothy E Shippen
Dec 22, 2020·PLoS Biology·Christopher E EllisonWeihuan Cao
Dec 19, 2021·Molecular Biology and Evolution·Iskander SaidDaniel A Barbash

❮ Previous
Next ❯

Datasets Mentioned

BETA
EY03241
GSE98981
DMU02279
AJ542581

Methods Mentioned

BETA
RNA-seq
transgenic
immunoprecipitation
ChIP
PCR

Software Mentioned

Imaris

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.