Key Role of Sequencing to Trace Hepatitis A Viruses Circulating in Italy During a Large Multi-Country European Foodborne Outbreak in 2013

PloS One
Roberto BruniItalian National Task Force on Hepatitis A

Abstract

Foodborne Hepatitis A Virus (HAV) outbreaks are being recognized as an emerging public health problem in industrialized countries. In 2013 three foodborne HAV outbreaks occurred in Europe and one in USA. During the largest of the three European outbreaks, most cases occurred in Italy (>1,200 cases as of March 31, 2014). A national Task Force was established at the beginning of the outbreak by the Ministry of Health. Mixed frozen berries were early demonstrated to be the source of infection by the identity of viral sequences in patients and in food. In the present study the molecular characterization of HAV isolates from 355 Italian cases is reported. Molecular characterization was carried out by PCR/sequencing (VP1/2A region), comparison with reference strains and phylogenetic analysis. A unique strain was responsible for most characterized cases (235/355, 66.1%). Molecular data had a key role in tracing this outbreak, allowing 110 out of the 235 outbreak cases (46.8%) to be recognized in absence of any other link. The data also showed background circulation of further unrelated strains, both autochthonous and travel related, whose sequence comparison highlighted minor outbreaks and small clusters, most of them unrecognized on ...Continue Reading

References

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Citations

Jan 14, 2017·Journal of Medical Microbiology·Gaia Scavia National Italian Task Force On Hepatitis A
Mar 2, 2017·Medicine·Maria Filomena GalloneCinzia Germinario
Nov 11, 2017·PloS One·Simone LaniniUNKNOWN Gruppo Laziale Sorveglianza Epatiti Virali (GLaSEV)
Jan 11, 2019·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Mayuko OdaKenji Sadamasu
Jun 18, 2017·Journal of Medical Virology·Angela CostantinoAnna Rita Ciccaglione
Jul 28, 2016·Current Opinion in Infectious Diseases·Miren Iturriza-Gomara, Sarah J O'Brien
Mar 7, 2017·Frontiers in Microbiology·David F Nieuwenhuijse, Marion P G Koopmans
Jul 15, 2017·BMC Infectious Diseases·Roberto BruniAnna Rita Ciccaglione
Jul 18, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Theresa EnkirchKatrin Leitmeyer
Feb 12, 2017·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Dirk WerberMirko Faber
Jan 23, 2021·Biomedicines·Claudia FerlitoRaffaele D'Amelio
Mar 6, 2021·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Maximilian RiessJosefine Lundberg Ederth
Oct 27, 2021·Food and Environmental Virology·Enrico PavoniMarina-Nadia Losio

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Datasets Mentioned

BETA
KF182323
KF706398

Methods Mentioned

BETA
genotyping
ISS
PCR

Software Mentioned

MEGA
BLAST

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