Key Transport and Ammonia Recycling Genes Involved in Aphid Symbiosis Respond to Host-Plant Specialization

G3 : Genes - Genomes - Genetics
Dohyup KimAllison K Hansen

Abstract

Microbes are known to influence insect-plant interactions; however, it is unclear if host-plant diet influences the regulation of nutritional insect symbioses. The pea aphid, Acyrthosiphon pisum, requires its nutritional endosymbiont, Buchnera, for the production of essential amino acids. We hypothesize that key aphid genes that regulate the nutritional symbioses respond to host-plant diet when aphids feed on a specialized (alfalfa) compared to a universal host-plant diet (fava), which vary in amino acid profiles. Using RNA-Seq and whole genome bisulfite sequencing, we measured gene expression and DNA methylation profiles for such genes when aphids fed on either their specialized or universal host-plant diets. Our results reveal that when aphids feed on their specialized host-plant they significantly up-regulate and/or hypo-methylate key aphid genes in bacteriocytes related to the amino acid metabolism, including glutamine synthetase in the GOGAT cycle that recycles ammonia into glutamine and the glutamine transporter ApGLNT1 Moreover, regardless of what host-plant aphids feed on we observed significant up-regulation and differential methylation of key genes involved in the amino acid metabolism and the glycine/serine metabolis...Continue Reading

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Citations

Jan 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Thomas E Smith, Nancy A Moran
Jul 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Honglin FengDavid T Thwaites
Dec 4, 2019·Trends in Ecology & Evolution·Stephanie S L Birnbaum, Patrick Abbot
Apr 9, 2021·G3 : Genes - Genomes - Genetics·Daniel Pers, Allison K Hansen

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Datasets Mentioned

BETA
PRJNA213008

Methods Mentioned

BETA
RNA-Seq
PCR
transmission electron microscopy
PCA

Software Mentioned

methylKit package
R
GSEA
Bismark
HISAT2
DESeq2
methylSig
Bowtie2

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