KGF-2 targets alveolar epithelia and capillary endothelia to reduce high altitude pulmonary oedema in rats.

Journal of Cellular and Molecular Medicine
Jun SheChunxue Bai

Abstract

High altitude pulmonary oedema (HAPE) severely affects non-acclimatized individuals and is characterized by alveolar flooding with protein-rich oedema as a consequence of blood-gas barrier disruption. Limited choice for prophylactic treatment warrants effective therapy against HAPE. Keratinocyte growth factor-2 (KGF-2) has shown efficiency in preventing alveolar epithelial cell DNA damages in vitro. In the current study, the effects of KGF-2 intratracheal instillation on mortality, lung liquid balance and lung histology were evaluated in our previously developed rat model of HAPE. We found that pre-treatment with KGF-2 (5 mg/kg) significantly decreased mortality, improved oxygenation and reduced lung wet-to-dry weight ratio by preventing alveolar-capillary barrier disruption demonstrated by histological examination and increasing alveolar fluid clearance up to 150%. In addition, KGF-2 significantly inhibited decrease of transendothelial permeability after exposure to hypoxia, accompanied by a 10-fold increase of Akt activity and inhibited apoptosis in human pulmonary microvascular endothelial cells, demonstrating attenuated endothelial apoptosis might contribute to reduction of endothelial permeability. These results showed the...Continue Reading

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Citations

Nov 28, 2013·Diagnostic Pathology·Jun SheChunxue Bai
Jan 24, 2014·American Journal of Respiratory Cell and Molecular Biology·Xiaocong FangXiangdong Wang
May 28, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Shih-Yu LeeTsu-Chung Chang
Jun 29, 2014·Respiratory Physiology & Neurobiology·Lin TongChunxue Bai
Dec 1, 2015·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Nana FengHuayin Li
Jul 16, 2015·The Clinical Respiratory Journal·Ruijuan SunErdan Dong
Aug 11, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ye GaoShengwei Jin
Nov 24, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Olivier BernardNicolas Dard
Nov 16, 2018·Frontiers in Genetics·Lawrence S Prince
Oct 16, 2018·Frontiers in Genetics·Tingting YuanStijn P De Langhe
May 24, 2020·Acta Biochimica Et Biophysica Sinica·Ailing Wu, Hai Song
Aug 6, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·S TenghaoM Xigang

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Methods Mentioned

BETA
acid titration
electron microscopy
Fluorescence
co-immunoprecipitation

Software Mentioned

SAS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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