KGF inhibits hypoxia-induced intestinal epithelial cell apoptosis by upregulating AKT/ERK pathway-dependent E-cadherin expression

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Yujiao CaiJie Mei

Abstract

Intestinal ischemia-reperfusion (I/R) causes direct cellular damage, and the potential injury to the mucosal structure and barrier function. Keratinocyte growth factor (KGF) is highly expressed in gastrointestinal tract and exerts beneficial effects for intestinal epithelial growth and maintenance. E-cadherin plays an important role in intestinal epithelium renewal. However, the regulatory role of KGF on E-cadherin levels and I/R-induced apoptosis remain to be explored. The present study aimed to identify the effect of KGF on E-cadherin expression and I/R-induced intestinal epithelial cell apoptosis. Caco2 cells were treated with KGF (100 ng/ml) for 0, 4, 8, 12, and 24 h under hypoxia or normoxia. An E-cadherin-knockdown model was successfully established by treatment with E-cadherin RNAi. Western blotting and immunofluorescence labeling were performed to assess E-cadherin expression. Levels of PI3K|[sol]|Akt/mitogen-activated protein kinases (MAPKs), phosphoinositide 3-kinase (PI3K|[sol]|Akt)/PI3K|[sol]|Akt pathway-related proteins, and apoptosis-related proteins were also detected by western blot. Finally, a rat model of acute intestinal I/R was established and treated with KGF. Hematoxylin-eosin (HE), terminal deoxynucleotid...Continue Reading

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Citations

Oct 5, 2019·Nature Communications·Fanny LebretonEkaterine Berishvili
Sep 4, 2020·International Journal of Environmental Research and Public Health·Jeong Mi KimJeong-Seok Choi
Oct 23, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nicole J EdwardsBenjamin Levi
Jan 20, 2021·International Journal of Radiation Biology·Xin ZhangYuhui Hao
Feb 10, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mengxi WangHong Lei

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