Ki-67 and condensins support the integrity of mitotic chromosomes through distinct mechanisms

Journal of Cell Science
Masatoshi TakagiNaoko Imamoto

Abstract

Although condensins play essential roles in mitotic chromosome assembly, Ki-67 (also known as MKI67), a protein localizing to the periphery of mitotic chromosomes, had also been shown to make a contribution to the process. To examine their respective roles, we generated a set of HCT116-based cell lines expressing Ki-67 and/or condensin subunits that were fused with an auxin-inducible degron for their conditional degradation. Both the localization and the dynamic behavior of Ki-67 on mitotic chromosomes were not largely affected upon depletion of condensin subunits, and vice versa. When both Ki-67 and SMC2 (a core subunit of condensins) were depleted, ball-like chromosome clusters with no sign of discernible thread-like structures were observed. This severe defective phenotype was distinct from that observed in cells depleted of either Ki-67 or SMC2 alone. Our results show that Ki-67 and condensins, which localize to the external surface and the central axis of mitotic chromosomes, respectively, have independent yet cooperative functions in supporting the structural integrity of mitotic chromosomes.

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Citations

May 18, 2020·Proceedings of the National Academy of Sciences of the United States of America·Christian F NielsenDamien F Hudson
May 2, 2018·The Journal of Cell Biology·Kazuhiro MaeshimaDamien F Hudson
Jan 17, 2020·JCI Insight·Kristen M GlisinskiChristina E Barkauskas
Apr 11, 2019·Essays in Biochemistry·Eloise I Prieto, Kazuhiro Maeshima
Apr 11, 2021·Seminars in Cell & Developmental Biology·James R PaulsonWilliam C Earnshaw
Aug 7, 2021·Proceedings of the National Academy of Sciences of the United States of America·Osama GarwainPaul D Kaufman
Aug 11, 2021·Open Biology·Lucy RemnantWilliam C Earnshaw
Nov 17, 2021·Biochemical Society Transactions·Konstantinos Stamatiou, Paola Vagnarelli

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