Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.

Vaccine
Xi LiTim R Mosmann

Abstract

Although previous studies have found minimal changes in CD4 T cell responses after vaccination of adults with trivalent inactivated influenza vaccine, daily sampling and monitoring of the proliferation marker Ki-67 have now been used to reveal that a substantial fraction of influenza-specific CD4 T cells respond to vaccination. At 4-6 days after vaccination, there is a sharp rise in the numbers of Ki-67-expressing PBMC that produce IFNγ, IL-2 and/or TNFα in vitro in response to influenza vaccine or peptide. Ki-67(+) cell numbers then decline rapidly, and 10 days after vaccination, both Ki-67(+) and overall influenza-specific cell numbers are similar to pre-vaccination levels. These results provide a tool for assessing the quality and quantity of CD4 T cell responses to different influenza vaccines, and raise the possibility that the anti-influenza T cell memory response may be qualitatively altered by vaccination, even if the overall memory cell numbers do not change significantly.

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Citations

Aug 2, 2012·The Journal of Experimental Medicine·Andrea J Sant, Andrew McMichael
Feb 18, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Tim R MosmannGaurav Sharma
Oct 22, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Katja KleinsteuberBruce D Walker
Aug 31, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ramin Sedaghat HeratiE John Wherry
Dec 24, 2018·Scientific Reports·David A G SkibinskiJohn E Connolly
Dec 10, 2019·The Journal of Clinical Investigation·Aleks GuvenelChristopher Chiu
Feb 2, 2021·PLoS Pathogens·Diana Cortés-SelvaKeke C Fairfax
Jun 25, 2018·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Munyemana Jean BoscoFeng Wang

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