Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development

Animal Science Journal = Nihon Chikusan Gakkaihō
Akiyoshi EgashiraTakashi Kuramoto

Abstract

This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1- and 2-cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4-cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2- to 4-cell arrested embryos and reduce the developmental competence to blastocyst.

References

Sep 24, 2005·Molecular Biology of the Cell·Noriko Tokai-NishizumiTadashi Yamamoto
Oct 17, 2007·The Journal of Reproduction and Development·Satoshi Kishigami, Teruhiko Wakayama
May 10, 2011·Fertility and Sterility·Hee-Jun ChiSung-Il Roh

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