Kidins220/ARMS binds to the B cell antigen receptor and regulates B cell development and activation

The Journal of Experimental Medicine
Gina J FialaWolfgang W A Schamel

Abstract

B cell antigen receptor (BCR) signaling is critical for B cell development and activation. Using mass spectrometry, we identified a protein kinase D-interacting substrate of 220 kD (Kidins220)/ankyrin repeat-rich membrane-spanning protein (ARMS) as a novel interaction partner of resting and stimulated BCR. Upon BCR stimulation, the interaction increases in a Src kinase-independent manner. By knocking down Kidins220 in a B cell line and generating a conditional B cell-specific Kidins220 knockout (B-KO) mouse strain, we show that Kidins220 couples the BCR to PLCγ2, Ca(2+), and extracellular signal-regulated kinase (Erk) signaling. Consequently, BCR-mediated B cell activation was reduced in vitro and in vivo upon Kidins220 deletion. Furthermore, B cell development was impaired at stages where pre-BCR or BCR signaling is required. Most strikingly, λ light chain-positive B cells were reduced sixfold in the B-KO mice, genetically placing Kidins220 in the PLCγ2 pathway. Thus, our data indicate that Kidins220 positively regulates pre-BCR and BCR functioning.

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Citations

Mar 26, 2016·Frontiers in Cellular Neuroscience·Joachim Scholz-Starke, Fabrizia Cesca
Nov 22, 2016·Genes, Chromosomes & Cancer·Kenichi SakamotoAtsushi Sato
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Mar 26, 2021·Frontiers in Cell and Developmental Biology·Kaihui ZhangYiyuan Yuan

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Methods Mentioned

BETA
nucleotide exchange
transgenic
flow cytometry
immunoprecipitations
ELISA
acetylation
FCS

Software Mentioned

MaxQuant
ImageQuant TL
GraphPad Prism

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