Jan 5, 2019

Kidney and organoid single-cell transcriptomics: the end of the beginning

Pediatric Nephrology : Journal of the International Pediatric Nephrology Association
Parker C Wilson, Benjamin D Humphreys

Abstract

Single-cell RNA sequencing (scRNA-seq) technologies are increasingly being applied to reveal cellular heterogeneity in kidney development and disease. In just the last year, multiple scRNA-seq datasets have been generated from kidney organoids, developing mouse and human kidney, adult kidney, and kidney cancer. The data generated enables a much deeper understanding of biological processes within and between cells. It has also elucidated unforeseen cell lineage relationships, defined the presence of off-target cell types in kidney organoids, and revealed a diverse inflammatory response in a human kidney allograft undergoing rejection. This review summarizes the recent rapid progress in scRNA-seq of the kidney and outlines future directions for single-cell technologies as applied to the kidney.

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Mentioned in this Paper

Sequence Determinations, RNA
Sequence Determinations
Kidney
Organoids
Inflammatory Response
Cell Separation
Laboratory mice

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