Kif5 regulates mitochondrial movement, morphology, function and neuronal survival

Molecular and Cellular Neurosciences
Diepiriye G IworimaGordon L Rintoul

Abstract

Due to the unique architecture of neurons, trafficking of mitochondria throughout processes to regions of high energetic demand is critical to sustain neuronal health. It has been suggested that compromised mitochondrial trafficking may play a role in neurodegenerative diseases. We evaluated the consequences of disrupted kif5c-mediated mitochondrial trafficking on mitochondrial form and function in primary rat cortical neurons. Morphological changes in mitochondria appeared to be due to remodelling, a phenomenon distinct from mitochondrial fission, which resulted in punctate-shaped mitochondria. We also demonstrated that neurons displaying punctate mitochondria exhibited relatively decreased ROS and increased cellular ATP levels using ROS-sensitive GFP and ATP FRET probes, respectively. Somewhat unexpectedly, neurons overexpressing the dominant negative form of kif5c exhibited enhanced survival following excitotoxicity, suggesting that the impairment of mitochondrial trafficking conferred some form of neuroprotection. However, when neurons were exposed to H2O2, disruption of kif5c exacerbated cell death indicating that the effect on cell viability was dependent on the mode of toxicity. Our results suggest a novel role of kif5c....Continue Reading

References

Jan 1, 1991·Annual Review of Neuroscience·R B Vallee, G S Bloom
Jan 1, 1996·Annual Review of Pharmacology and Toxicology·N A Simonian, J T Coyle
Aug 1, 1997·Neuroscience Letters·R E MischelD M Ferriero
Aug 10, 2000·European Journal of Biochemistry·J B SchulzJ Dichgans
Feb 13, 2001·Trends in Biochemical Sciences·V P Skulachev
Mar 10, 2001·The Journal of Cell Biology·K J VerheyB Margolis
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·H MikiN Hirokawa
Feb 21, 2002·The Journal of Cell Biology·Steven P GrossEric F Wieschaus
Mar 18, 2003·The Journal of Clinical Investigation·Jeffrey A Klein, Susan L Ackerman
Jan 15, 2004·The Journal of Biological Chemistry·George T HansonS James Remington
Jan 23, 2004·Nature·Yuriy KirichokDavid E Clapham
Feb 27, 2004·The Journal of Biological Chemistry·Lee A LigonErika L F Holzbaur
May 4, 2004·Methods : a Companion to Methods in Enzymology·Brian DalbyValentina C Ciccarone
Oct 7, 2004·Current Opinion in Neurobiology·Nobutaka Hirokawa, Reiko Takemura
Feb 16, 2005·Nature Reviews. Neuroscience·Nobutaka Hirokawa, Reiko Takemura
May 19, 2005·The Journal of Biological Chemistry·Hsiuchen ChenDavid C Chan
Oct 14, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Latha M MalaiyandiIan J Reynolds
Apr 12, 2006·Journal of Neurochemistry·Santina A ZanelliNina J Solenski
May 30, 2006·Biochimica Et Biophysica Acta·Erica Chevalier-Larsen, Erika L F Holzbaur
Jul 28, 2006·Neurotoxicology·Michael TeepkerHelmut Vedder
Apr 21, 2009·Nature Neuroscience·Yoshiyuki Konishi, Mitsutoshi Setou
Jun 2, 2009·The International Journal of Biochemistry & Cell Biology·Xingguo Liu, György Hajnóczky
Sep 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hiromi ImamuraHiroyuki Noji
Sep 15, 2009·Biochimica Et Biophysica Acta·Arezu Jahani-AslRuth S Slack
Sep 15, 2009·Biochimica Et Biophysica Acta·Gordon L Rintoul, Ian J Reynolds
Jan 1, 1945·The Journal of Experimental Medicine·A Claude, E F Fullam
Aug 27, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yujiro Shidara, Peter J Hollenbeck
Apr 2, 2011·The Journal of Biological Chemistry·Kieran Brickley, F Anne Stephenson
May 1, 2012·Neuron·Armen J Moughamian, Erika L F Holzbaur
May 24, 2012·Journal of Cell Science·William M Saxton, Peter J Hollenbeck

❮ Previous
Next ❯

Citations

Jul 22, 2020·Physical Review. E·A B Fernández CasafuzL Bruno
May 13, 2018·Bioscience Reports·María Cecilia De RossiLuciana Bruno
Feb 14, 2021·Cells·Sara Marmolejo-Martínez-ArteseroDavid Romeo-Guitart
Mar 30, 2021·Frontiers in Psychiatry·Raymond A ClarkeValsamma Eapen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.