PMID: 8588746Dec 1, 1995Paper

Killer-toxin-resistant kre12 mutants of Saccharomyces cerevisiae: genetic and biochemical evidence for a secondary K1 membrane receptor

Archives of Microbiology
M J Schmitt, P Compain

Abstract

The Saccharomyces cerevisiae killer toxin K1 is a secreted alpha/beta-heterodimeric protein toxin that kills sensitive yeast cells in a receptor-mediated two-stage process. The first step involves toxin binding to beta-1,6-D-glucan-components of the outer yeast cell surface; this step is blocked in yeast mutants bearing nuclear mutations in any of the KRE genes whose products are involved in synthesis and/or assembly of cell wall beta-D-glucans. After binding to the yeast cell wall, the killer toxin is transferred to the cytoplasmic membrane, subsequently leading to cell death by forming lethal ion channels. In an attempt to identify a secondary K1 toxin receptor at the plasma membrane level, we mutagenized sensitive yeast strains and isolated killer-resistant (kre) mutants that were resistant as spheroplasts. Classical yeast genetics and successive back-crossings to sensitive wild-type strains indicated that this toxin resistance is due to mutation(s) in a single chromosomal yeast gene (KRE12), rendering kre12 mutants incapable of binding significant amounts of toxin to the membrane. Since kre12 mutants showed normal toxin binding to the cell wall, but markedly reduced membrane binding, we isolated and purified cytoplasmic mem...Continue Reading

References

Mar 1, 1978·Canadian Journal of Microbiology·K Al-Aidroos, H Bussey
Feb 10, 1976·Biochemistry·G F Ames, K Nikaido
Jan 1, 1975·Methods in Cell Biology·R K Mortimer, D C Hawthorne
Oct 1, 1991·Molecular Microbiology·D J Tipper, M J Schmitt
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B MartinacC Kung
Jan 1, 1991·Molecular and Cellular Biology·H Zhu, H Bussey
Oct 1, 1991·Molecular Microbiology·H Bussey
May 1, 1990·The Journal of Cell Biology·C BooneH Bussey
Sep 1, 1990·Molecular and Cellular Biology·M J Schmitt, D J Tipper
Apr 1, 1983·Journal of Bacteriology·K Hutchins, H Bussey
Jul 27, 1983·Biochimica Et Biophysica Acta·R SchmidtB Jacobson
Jan 20, 1995·Molecular & General Genetics : MGG·M J Schmitt
Aug 1, 1989·Applied and Environmental Microbiology·Hong Zhu, Howard Bussey

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Citations

Aug 8, 2002·FEMS Microbiology Reviews·Manfred J Schmitt, Frank Breinig
Oct 1, 2003·Fungal Genetics and Biology : FG & B·Kathelijne K A FerketKarin Thevissen
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·D J YunR A Bressan
May 2, 2000·Applied and Environmental Microbiology·A SantosJ M Peinado
Feb 21, 2002·Cell·Frank BreinigManfred J Schmitt
Jan 5, 2002·Microbiological Research·D MarquinaJ M Peinado
Sep 29, 2006·Microbiology·Masahiko MiyamotoTadazumi Komiyama
Dec 3, 1999·Applied and Environmental Microbiology·K ThevissenW F Broekaert
Jun 8, 2001·Cell·F SestiS A Goldstein
Jul 1, 1997·Clinical Microbiology Reviews·W MaglianiL Polonelli

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