Killing and lysis of gram-negative bacteria through the synergistic effect of hydrogen peroxide, ascorbic acid, and lysozyme.

Journal of Bacteriology
T E Miller

Abstract

A mixture of hydrogen peroxide and ascorbic acid has been found to generate an antibacterial mechanism which is active against gram-negative bacteria. It results in bacterial death and renders the organism sensitive to lysis by lysozyme. Under the conditions used, horseradish peroxidase did not augment the antibacterial effect. It is suggested that the effector mechanism involves the generation of short-lived free radicals which disturb the integrity of the cell wall. This effect alone might kill bacteria by interfering with selective permeability, but in the presence of lysozyme a further bactericidal activity is accomplished by complete disruption of the cell. It is proposed that a transient antibacterial system such as that described could exist within phagocytic cells. Free radicals would be formed through the interaction of certain oxidizable substances and hydrogen peroxide, which is produced during the enhanced metabolic activity that accompanies ingestion of bacteria. Such a system would help to explain why macrophages, which are apparently devoid of preformed bactericidins, are nonetheless very efficient in killing most phagocytosed bacteria.

References

Oct 1, 1966·The Journal of Experimental Medicine·R V BlandenF M Collins
Dec 1, 1967·The Journal of Experimental Medicine·S J Klebanoff
Jun 1, 1968·Journal of Bacteriology·S J Klebanoff
Jan 1, 1965·Annual Review of Microbiology·J G Hirsch
Jan 1, 1955·Acta Pathologica Et Microbiologica Scandinavica·Y ERICSSON, H LUNDBECK
Jan 1, 1955·Acta Pathologica Et Microbiologica Scandinavica·Y ERICSSON, H LUNDBECK
Oct 1, 1956·Biochimica Et Biophysica Acta·R REPASKE
Jun 1, 1957·Bacteriological Reviews·M R SALTON
Apr 21, 1961·Annals of the New York Academy of Sciences·W V ROBERTSON
Jan 1, 1964·Angewandte Chemie·P JOLLES
Jul 1, 1964·The Journal of Experimental Medicine·G B MACKANESS
Jul 1, 1948·The Journal of Infectious Diseases·W J NUNGESTER, A M AMES

❮ Previous
Next ❯

Citations

Sep 1, 1978·CRC Critical Reviews in Toxicology·M N Walters, J M Papadimitriou
Jan 1, 1977·Scandinavian Journal of Infectious Diseases·J LudvigssonG Tibbling
Jul 1, 1975·Journal of Dairy Science·F L Schanbacher, K L Smith
Jan 1, 1986·Histochemistry·R T BriggsM J Karnovsky
Apr 1, 1975·Archives of Disease in Childhood·M XanthouN Matsaniotis
Apr 1, 1976·Journal of Dental Research·I D Mandel
Jan 1, 1991·Free Radical Research Communications·T IshiiR Kido
Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·C F Nathan
Jan 15, 1990·FEBS Letters·P WashkoM Levine
Dec 1, 1987·Zentralblatt für Veterinärmedizin. Reihe A·E Wiśniewski, K Kuźma
Dec 1, 1970·The Journal of Membrane Biology·D S Feingold
Dec 1, 1979·Developmental Medicine and Child Neurology·M Adinolfi, A Glynn
Feb 1, 1977·Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology·D Bratlid, K Bovre
Jan 1, 1988·Critical Reviews in Food Science and Nutrition·V A Proctor, F E Cunningham
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y WangM Levine
Jan 1, 1981·The Histochemical Journal·M J KarnovskyM L Karnovsky
Aug 24, 2011·Journal of Medicinal Food·Noori S Al-WailiAhmad A Al Ghamdi
Jul 1, 1981·Issues in Comprehensive Pediatric Nursing·R D Jeffries
Feb 9, 2010·Analytical Chemistry·Pavel A LevashovAndrey V Levashov
Sep 1, 1982·Applied and Environmental Microbiology·N J Ragab-Depre
Jan 31, 1984·Biochimica Et Biophysica Acta·B G BolscherR Wever
Nov 1, 1974·Infection and Immunity·D B Drath, M L Karnovsky
Aug 1, 1975·Infection and Immunity·L StankovaR H Bigley
Jan 1, 1972·Antimicrobial Agents and Chemotherapy·L R DeChateletC E McCall
Apr 1, 1972·Infection and Immunity·L R DeChateletM R Cooper
Oct 10, 1974·Prostaglandins·R V PanganamalaD G Cornwell
Mar 1, 1975·Medical Hypotheses·P W Stacpoole
Jun 1, 1973·The Journal of Pediatrics·A S Goldman, C W Smith
Dec 17, 2016·ACS Applied Materials & Interfaces·Jin GuPeng Yang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.