Killing bacteria within biofilms by sustained release of tetracycline from triple-layered electrospun micro/nanofibre matrices of polycaprolactone and poly(ethylene-co-vinyl acetate)

Drug Delivery and Translational Research
Nour AlhuseinAlbert Bolhuis

Abstract

We report the controlled release of the antibiotic tetracycline (tet) HCl from a triple-layered electrospun matrix consisting of a central layer of poly(ethylene-co-vinyl acetate (PEVA) sandwiched between outer layers of poly-ε-caprolactone (PCL). These micro/nanofibre layers with tet successfully encapsulated (essentially quantitatively at 3 and 5 % w/w) in each layer, efficiently inhibited the growth of a panel of bacteria, including clinical isolates, as shown by a modified Kirby-Bauer disc assay. Furthermore, they demonstrated high biological activity in increasingly complex models of biofilm formation (models that are moving closer to the situation in a wound) by stopping biofilm formation, by killing preformed biofilms and killing mature, dense biofilm colonies of Staphylococcus aureus MRSA252. Tet is clinically useful with potential applications in wound healing and especially in complicated skin and skin-structure infections; electrospinning provides good encapsulation efficiency of tet within PCL/PEVA/PCL polymers in micro/nanofibre layers which display sustained antibiotic release in formulations that are anti-biofilm.

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Citations

Dec 1, 2013·Drug Delivery and Translational Research·Nour AlhuseinPaul A De Bank
Jun 9, 2015·Journal of Pharmaceutical Sciences·Young Ju SonHyuk Sang Yoo
Oct 16, 2014·Molecular Pharmaceutics·Felipe L LopezGareth R Williams
Feb 28, 2015·Advanced Healthcare Materials·Mohammad NorouziMasoud Soleimani
Feb 20, 2014·Advanced Drug Delivery Reviews·Meng-Hua XiongJun Wang
Nov 1, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ritu GoyalJoachim Kohn
Aug 16, 2014·Journal of Inorganic Biochemistry·Michael L BeetonAlbert Bolhuis
Apr 16, 2020·Antibiotics·Catarina S MirandaHelena P Felgueiras
Dec 23, 2017·Asian Pacific Journal of Tropical Medicine·Nguyen Thi Mai PhuongAlbert Bolhuis
Dec 28, 2018·Pharmaceutics·Rajan Sharma BhattaraiSarit Bhaduri
Sep 19, 2018·Materials·Eliška ČíkováMária Omastová
Dec 28, 2018·Pharmaceutics·Laura Modica de MohacBahijja Tolulope Raimi-Abraham
Mar 19, 2021·Saudi Journal of Biological Sciences·Phuong T M NguyenAlbert Bolhuis
Jul 25, 2021·Materials·Dorota KidaBożena Karolewicz

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