Killing of Leishmania donovani amastigotes by poly ICLC in hamsters

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
V BhakuniR K Maheshwari

Abstract

In vitro as well as in vivo studies suggest that cytokine-induced synthesis of nitric oxide (NO) from L-arginine is a major effector mechanism against intracellular pathogens. In this study, we demonstrate that golden hamsters infected with Leishmania donovani amastigotes upon treatment with polyinosinic-polycytidylic acid stabilized with polylysine and carboxymethylcellulose (poly ICLC), a potent interferon inducer and immune enhancer, in combination with L-arginine, develop the capacity to eliminate intracellular pathogens. This antileishmanial activity of poly ICLC was suppressed by N w nitro-L-arginine (N w NLA), an inhibitor of inducible NO synthase. Furthermore, prolonged treatment of infected animals with L-arginine alone for 5 days more after 5 day treatment with poly ICLC plus L-arginine increased the antileishmanial activity compared with 5 day treatment with poly ICLC plus L-arginine, suggesting that inducible NO synthase, once activated, produces NO for 5 days more. Our results suggest that an L-arginine-dependent, NO-mediated mechanism is probably responsible for the antileishmanial action of poly ICLC.

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Citations

Nov 28, 2007·Immunologic Research·Nanchaya Wanasen, Lynn Soong
Nov 5, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·V BhakuniR K Maheshwari
Jul 1, 1997·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·A AwasthiR K Maheshwari
May 19, 2000·Antimicrobial Agents and Chemotherapy·A C SmithS L Croft
Jul 26, 2005·Infection and Immunity·Blaise DondjiDiane McMahon-Pratt
Oct 29, 2011·Molecular and Biochemical Parasitology·Rafael Balaña-FouceRosa M Reguera

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