Killing of naive T cells by CD95L-transfected dendritic cells (DC): in vivo study using killer DC-DC hybrids and CD4(+) T cells from DO11.10 mice

European Journal of Immunology
Masahiro KusuharaHiroyuki Matsue

Abstract

Dendritic cells (DC) play the dual task of initiating cellular immunity against potentially harmful foreign antigens (Ag), while maintaining immunological tolerance to self-Ag and environmental Ag. As an approach to induce Ag-specific suppression, we and others introduced CD95 ligand (L) cDNA into DC. The resulting "killer" DC delivered apoptotic signals, instead of activation signals, to primed CD4(+) T cells in vitro and induced Ag-specific immunosuppression in vivo. To study the impact of killer DC on naive T cells, the fate of Ag-reactive T cells and the extent of their depletion after killer DC treatment, we performed in vitro and in vivo reconstitution experiments using: (a) killer DC-DC hybrids created between CD95L-transduced XS106 DC clone (A/J origin) and splenic DC from BALB/c mice, (b) CD4(+) T cells isolated from DO11.10 transgenic mice (BALB/c background), and (c) OVA(323-339) peptide as relevant Ag. Ovalbumin (OVA)-pulsed killer DC-DC hybrids inhibited DO11.10 T cell activation triggered by conventional DC, instead of inducing their activation. Rapid apoptosis of T cells was observed after co-culture with OVA-pulsed killer DC-DC hybrids, but not with non-pulsed killer DC-DC hybrids or OVA-pulsed control DC-DC hyb...Continue Reading

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Citations

Jul 2, 2003·The Journal of Experimental Medicine·Vincent HurezCasey T Weaver
Mar 28, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ya-Hui ChuangBor-Luen Chiang
Dec 13, 2006·Clinical & Developmental Immunology·Alice LiAlan Escher
Jun 22, 2010·Expert Opinion on Therapeutic Targets·Dipender Gill, Peng H Tan
Mar 12, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ryan A Langlois, Kevin L Legge
Nov 13, 2004·Stem Cells·Osnat Bohana-Kashtan, Curt I Civin
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tom WolfeMatthias G von Herrath
May 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sabine HovesMartin Fleck

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