Killing of Schistosoma mansoni sporocysts by hemocytes from resistant Biomphalaria glabrata: role of reactive oxygen species

The Journal of Parasitology
U K HahnChristopher J Bayne

Abstract

The fate of Schistosoma mansoni (Trematoda) sporocysts in its molluscan host Biomphalaria glabrata (Gastropoda) is determined by circulating phagocytes (hemocytes). When the parasite invades a resistant snail, it is attacked and destroyed by hemocytes, whereas in a susceptible host it remains unaffected. We used 3 inbred strains of B. glabrata: 13-16-R1 and 10-R2, which are resistant to the PR-1 strain of S. mansoni, and M-line Oregon (MO), which is susceptible to PR-1. In an in vitro killing assay using plasma-free hemocytes from these strains, the rate of parasite killing corresponded closely to the rate by which S. mansoni sporocysts are killed in vivo. Hemocytes from resistant snails killed more than 80% of S. mansoni sporocysts within 48 hr, whereas sporocyst mortality in the presence of hemocytes from susceptible snails was <10%. Using this in vitro assay, we assessed the involvement of reactive oxygen species (ROS) produced by resistant hemocytes, during killing of S. mansoni sporocysts. Inhibition of NADPH oxidase significantly reduced sporocyst killing by 13-16-R1 hemocytes, indicating that ROS play an important role in normal killing. Reduction of hydrogen peroxide (H2O2) by including catalase in the killing assay inc...Continue Reading

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Citations

May 9, 2014·Proceedings. Biological Sciences·Yves MonéNatacha Kremer
Sep 9, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Adel FarghalyMarwa A Salama
Jul 19, 2014·Developmental and Comparative Immunology·C M Adema, E S Loker
May 31, 2012·The Journal of Parasitology·Michelle L Steinauer, Kaitlin M Bonner
Mar 3, 2004·Experimental Parasitology·Eric J SchottGerardo R Vasta
Mar 5, 2003·Molecular and Biochemical Parasitology·Nithya RaghavanMatty Knight
Nov 10, 2015·International Journal for Parasitology·Hannah F TavalireMichelle L Steinauer
Mar 31, 2011·International Journal for Parasitology·Benjamin MorgaIsabelle Arzul
Dec 20, 2008·Molecular and Biochemical Parasitology·Xiao-Jun WuTimothy P Yoshino
Nov 6, 2007·Developmental and Comparative Immunology·Judith E Humphries, Timothy P Yoshino
Dec 15, 2006·Developmental and Comparative Immunology·François GuillouChristine Coustau
Nov 13, 2004·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·A H Mahmoud, M Z Rizk
Aug 25, 2004·Developmental and Comparative Immunology·Y CartonM Poirie
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Dec 5, 2012·International Journal for Parasitology·Michael S BlouinChristopher J Bayne
May 15, 2009·Molecular and Biochemical Parasitology·Matty KnightChristopher J Bayne
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Apr 7, 2009·Brazilian Journal of Biology = Revista Brasleira De Biologia·S M AllegrettiE M Zanotti-Magalhães

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