Kindlin-2 is expressed in malignant mesothelioma and is required for tumor cell adhesion and migration

International Journal of Cancer. Journal International Du Cancer
Zhengwen AnHongquan Zhang

Abstract

Kindlin-2 is a novel integrin-interacting focal adhesion protein that belongs to the Kindlin family. Focal adhesion proteins control cytoskeleton dynamics and promote cancer cell growth, survival, migration and metastasis. Little is known, however, about expression of Kindlin-2 in association with human cancer. We now reveal high Kindlin-2 expression in malignant mesothelioma (MM) cell lines using an affinity-purified anti-Kindlin-2 antibody. Furthermore, we show by immunohistochemistry that Kindlin-2 is highly expressed in 92 of 102 (90%) MMs with epitheliod; sarcomatoid, biphasic and poorly differentiated morphologies. In addition, Kindlin-2 expression correlates to cell proliferation, suggesting a role for Kindlin-2 in tumor growth. We also detect increased expression of Kindlin-2 at the invasion front of tumors concurrent with increased expression of integrin-linked kinase, a Kindlin-binding protein. Besides the high expression of Kindlin-2 in pleural MMs, pleural metastases of lung adenocarcinoma also express large amounts of Kindlin-2, but not Kindlin-1. Notably, in vitro, when endogenous Kindlin-2 was knocked down with RNAi in MM cells, this impaired cell spreading, adhesion and migration. Overall, our study suggests tha...Continue Reading

References

Jan 1, 1992·Respiration; International Review of Thoracic Diseases·L TammilehtoK Mattson
Aug 1, 1989·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·P RuffieA Figueredo
Jan 1, 1984·Archives of Biochemistry and Biophysics·S A Weitzman, P Graceffa
Mar 30, 2001·The Journal of Biological Chemistry·J T DengM P Walsh
May 17, 2002·Current Biology : CB·A Craig MackinnonBenjamin D Williams
May 29, 2002·The Biochemical Journal·Andrea MurányiDavid J Hartshorne
Apr 11, 2003·The Journal of Biological Chemistry·Armelle A TroussardShoukat Dedhar
Nov 25, 2003·The Journal of Biological Chemistry·Susanne KloekerMary C Beckerle
Dec 12, 2003·Clinical Lung Cancer·Adi F Gazdar, Michele Carbone
Jan 30, 2004·Cancer Cell·Clara TanShoukat Dedhar
Feb 6, 2004·The Journal of Cell Biology·Hiroshi AkazawaIssei Komuro
Jan 5, 2005·Nature Reviews. Cancer·Gregory HanniganShoukat Dedhar
Feb 11, 2005·Journal of Cell Science·Chuanyue Wu
May 25, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Simon Fox, Arun Dharmarajan
Aug 1, 2006·Experimental Cell Research·Siegfried UssarMarkus Moser
Apr 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Filippo AcconciaRakesh Kumar
Dec 1, 2007·Interactive Cardiovascular and Thoracic Surgery·Stefan B WatzkaMichael R Müller
Feb 19, 2008·Nature Medicine·Markus MoserReinhard Fässler
May 17, 2008·Genes & Development·Eloi MontanezReinhard Fässler
May 29, 2008·Molecular Cancer Research : MCR·Xiaohua Shi, Chuanyue Wu
Jul 10, 2008·BMC Cell Biology·James J DowlingEva L Feldman
Mar 21, 2009·Blood·Shigenori HondaToshiyuki Miyata

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Citations

Dec 6, 2012·Cancer Letters·Ting ZhaoHongquan Zhang
Oct 12, 2012·PloS One·Hisashi KatoSanford J Shattil
Jan 23, 2013·FEBS Letters·Jianchao GaoHongquan Zhang
May 31, 2011·The Journal of Thoracic and Cardiovascular Surgery·Stefan B WatzkaMichael R Müller
Sep 24, 2010·The Journal of Investigative Dermatology·Yinghong HeCristina Has
Jan 27, 2015·Pathology, Research and Practice·Yong-Sheng GeGe-Liang Xu
Mar 14, 2013·International Journal of Cancer. Journal International Du Cancer·Yu YuHongquan Zhang
Mar 4, 2014·Biochemical and Biophysical Research Communications·Caixia RenHongquan Zhang
Jan 6, 2016·Journal of Cell Science·Emanuel RognoniReinhard Fässler
Sep 25, 2018·Cell Death Discovery·Jun YuHuilong Chen
Jun 1, 2013·Journal of the American Society of Nephrology : JASN·Xiaofan WeiFan Fan Hou
Oct 3, 2019·Scientific Reports·Lydon Wainaina NyamburaPeter Walden
Jan 24, 2018·Journal of Cell Science·Yuqing LiuHongquan Zhang
Oct 27, 2014·Oncotarget·Ibtissem DjaafriSamia Mourah
Oct 4, 2017·Journal of Experimental & Clinical Cancer Research : CR·Jie LinAimin Huang
Nov 15, 2013·American Journal of Physiology. Renal Physiology·Xiaofan WeiHongquan Zhang
Oct 26, 2016·Molecular Medicine Reports·Fa-Min ZengEn-Min Li
Apr 15, 2017·The Journal of Cell Biology·Xiaofan WeiHongquan Zhang
Jun 13, 2021·Cell Death & Disease·Xiaochun ChiHongquan Zhang

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