May 20, 2016

Kinematic analysis of cell lineage reveals coherent and robust mechanical deformation patterns in zebrafish gastrulation

BioRxiv : the Preprint Server for Biology
David Pastor-EscuredoMaria J. Ledesma-Carbayo

Abstract

Digital cell lineages reconstructed from 3D+time imaging data provide unique information to unveil mechanical cues and their role in morphogenetic processes. Our methodology based on a kinematic analysis of cell lineage data reveals deformation patterns and quantitative morphogenetic landmarks for a new type of developmental table. The characteristic spatial and temporal length scales of mechanical deformation patterns derived from a continuous approximation of cell displacements indicate a compressible fluid-like behavior of zebrafish gastrulating tissues. The instantaneous deformation rate at the mesoscopic level of the cell's neighborhood is spatially and temporally heterogeneous. The robustness of mechanical patterns results from their cumulative history along cell trajectories. Unsupervised classification of mechanical descriptor profiles was used to assess the homogeneity of biomechanical cues in cell populations. Further clustering of cell trajectories according to their cumulative mesoscopic biomechanical history during gastrulation revealed ordered and coherent spatiotemporal patterns comparable to that of the embryonic fate map.

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Mentioned in this Paper

Embryo
Patterns
Displacement - Action
Classification
Spatial Distribution
Cell-cell Adhesion Involved in Gastrulation
Kinematics
Digit Structure
Body Fluids
Zebrafish

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