Kinesin-2 and Apc function at dendrite branch points to resolve microtubule collisions

Cytoskeleton
Alexis T WeinerMelissa M Rolls

Abstract

In Drosophila neurons, kinesin-2, EB1 and Apc are required to maintain minus-end-out dendrite microtubule polarity, and we previously proposed they steer microtubules at branch points. Motor-mediated steering of microtubule plus ends could be accomplished in two ways: 1) by linking a growing microtubule tip to the side of an adjacent microtubule as it navigates the branch point (bundling), or 2) by directing a growing microtubule after a collision with a stable microtubule (collision resolution). Using live imaging to distinguish between these two mechanisms, we found that reduction of kinesin-2 did not alter the number of microtubules that grew along the edge of the branch points where stable microtubules are found. However, reduction of kinesin-2 or Apc did affect the number of microtubules that slowed down or depolymerized as they encountered the side of the branch opposite to the entry point. These results are consistent with kinesin-2 functioning with Apc to resolve collisions. However, they do not pinpoint stable microtubules as the collision partner as stable microtubules are typically very close to the membrane. To determine whether growing microtubules were steered along stable ones after a collision, we analyzed the b...Continue Reading

References

Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P W BaasG A Banker
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·L BerruetaB E Bierer
Mar 26, 2002·Nature Cell Biology·Takeshi JimboTetsu Akiyama
Mar 7, 2006·Cell Motility and the Cytoskeleton·Nina KarpovaAlain Debec
Oct 14, 2006·Journal of Cell Science·Francesca Bartolini, Gregg G Gundersen
Sep 12, 2007·The Journal of Cell Biology·Kenneth A Myers, Peter W Baas
Nov 16, 2007·Molecular Biology of the Cell·Christiane Wiese
Feb 13, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·P ThévenazM Unser
Apr 29, 2008·Neuron·Iris KochHermann Aberle
Aug 1, 2008·Molecular Biology of the Cell·Michelle C StoneMelissa M Rolls
Jan 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ram DixitErika L F Holzbaur
Feb 9, 2010·Current Biology : CB·Lukas C KapiteinCasper C Hoogenraad
Nov 5, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mei LiuPeter W Baas
May 11, 2011·Developmental Neurobiology·Peter W Baas, Shen Lin
Jun 16, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Patricia R GoodwinPeter Juo
Jun 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Li ChenMelissa M Rolls
Oct 5, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shen LinPeter W Baas
Nov 1, 2012·Neural Development·Sarah E HillMelissa M Rolls
Oct 18, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Elliott B MerriamErik W Dent
Jan 28, 2014·Current Biology : CB·Harinath DoodhiAnna Akhmanova
Jan 28, 2014·Current Biology : CB·Yalei ChenWilliam O Hancock
Feb 11, 2014·Current Biology : CB·Sebastian P MaurerThomas Surrey
Feb 20, 2015·The Journal of Experimental Biology·Melissa M Rolls, Timothy J Jegla

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Citations

Feb 13, 2019·Journal of Neurotrauma·Mihir V PatelBonnie L Firestein
Apr 1, 2018·G3 : Genes - Genomes - Genetics·Alexis T WeinerMelissa M Rolls
Jul 19, 2018·Nature Communications·Olga I KahnCasper C Hoogenraad
Nov 2, 2016·Molecular Biology of the Cell·Kavitha RaoMelissa M Rolls
Feb 21, 2019·Developmental Cell·Kristina N Schaefer, Mark Peifer
May 11, 2021·Frontiers in Molecular Biosciences·Xiuyan YangShaoyong Lu
Dec 9, 2021·The Journal of Cell Biology·Helen E FosterAndrew P Carter

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