Kinesin 5-independent poleward flux of kinetochore microtubules in PtK1 cells.

The Journal of Cell Biology
Lisa A CameronE D Salmon

Abstract

Forces in the spindle that align and segregate chromosomes produce a steady poleward flux of kinetochore microtubules (MTs [kMTs]) in higher eukaryotes. In several nonmammalian systems, flux is driven by the tetrameric kinesin Eg5 (kinesin 5), which slides antiparallel MTs toward their minus ends. However, we find that the inhibition of kinesin 5 in mammalian cultured cells (PtK1) results in only minor reduction in the rate of kMT flux from approximately 0.7 to approximately 0.5 microm/min, the same rate measured in monopolar spindles that lack antiparallel MTs. These data reveal that the majority of poleward flux of kMTs in these cells is not driven by Eg5. Instead, we favor a polar "pulling-in" mechanism in which a depolymerase localized at kinetochore fiber minus ends makes a major contribution to poleward flux. One candidate, Kif2a (kinesin 13), was detected at minus ends of fluxing kinetochore fibers. Kif2a remains associated with the ends of K fibers upon disruption of the spindle by dynein/dynactin inhibition, and these K fibers flux.

References

Jul 1, 1992·The Journal of Cell Biology·K L McDonaldJ R McIntosh
Mar 1, 1991·The Journal of Cell Biology·K E Sawin, T J Mitchison
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T L Hill
Oct 29, 1981·Nature·R L Margolis, L Wilson
Nov 1, 1995·The Journal of Cell Biology·Y ZhaiG G Borisy
May 16, 1998·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·B F McEwenA B Heagle
Aug 15, 1998·Trends in Cell Biology·C L Rieder, E D Salmon
Sep 14, 2002·Science·George H Patterson, Jennifer Lippincott-Schwartz
Dec 12, 2002·Molecular Biology of the Cell·Jennifer S TirnauerTimothy J Mitchison
Jun 5, 2003·Angewandte Chemie·Srinivas HothaTarun M Kapoor
Sep 3, 2003·Journal of Microscopy·G Danuser, C M Waterman-Storer
Mar 25, 2004·Nature Cell Biology·Wei Chen, Dahong Zhang
Aug 18, 2004·The Journal of Cell Biology·Jedidiah Gaetz, Tarun M Kapoor
Sep 24, 2004·Molecular Biology of the Cell·T J MitchisonT M Kapoor
Oct 8, 2004·Molecular Biology of the Cell·James R LaFountainDouglas J LaFountain
Nov 9, 2004·Current Biology : CB·Mimi Shirasu-HizaTimothy J Mitchison
Dec 8, 2004·The Journal of Cell Biology·David T MiyamotoTimothy J Mitchison
Dec 14, 2004·Current Biology : CB·Daniela CiminiE D Salmon
Mar 15, 2005·Journal of Cell Science·Gregory C RogersDavid J Sharp
Apr 28, 2005·Current Biology : CB·E D Salmon
Oct 26, 2005·Current Biology : CB·Neil J GanemDuane A Compton
Nov 24, 2005·Current Biology : CB·Gohta GoshimaRonald D Vale

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Citations

Apr 19, 2008·Protoplasma·Arthur ForerTim Spurck
Jul 18, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·António J Pereira, Helder Maiato
Oct 16, 2007·Nature Cell Biology·Ge YangTarun M Kapoor
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Benjamin R HoughtalingTarun M Kapoor
Aug 3, 2007·Molecular Biology of the Cell·Nick P Ferenz, Patricia Wadsworth
Jan 23, 2009·Molecular Biology of the Cell·Rania S RizkClaire E Walczak
Jan 23, 2009·Molecular Biology of the Cell·Ingrid Brust-MascherJonathan M Scholey
Jan 30, 2010·Molecular Biology of the Cell·Nan MaPatricia Wadsworth
Dec 2, 2011·Molecular Biology of the Cell·William T SilkworthDaniela Cimini
Mar 15, 2013·Molecular Biology of the Cell·Jessica Ferraro-GideonArthur Forer
Jul 24, 2013·Annual Review of Cell and Developmental Biology·Claire E WalczakRyoma Ohi
Dec 19, 2006·Cell Division·Megan T ValentineSteven M Block
Apr 9, 2009·Journal of Cell Science·Chang-Young JangGuowei Fang
Jul 16, 2014·The Journal of Cell Biology·Mary Williard EltingSophie Dumont
Aug 20, 2008·The Journal of Cell Biology·Marianne UtengThomas Surrey
May 15, 2013·The Journal of Cell Biology·Gul Civelekoglu-ScholeyDaniela Cimini
Jul 8, 2009·The Journal of Cell Biology·Irina MatosHelder Maiato
Sep 30, 2015·Proceedings of the National Academy of Sciences of the United States of America·Edward J BaniganAndrea J Liu
Dec 19, 2006·Current Opinion in Cell Biology·Benjamin H Kwok, Tarun M Kapoor
Apr 18, 2009·Cell Motility and the Cytoskeleton·Lynne CassimerisBruce Carney
Dec 6, 2008·Biology of the Cell·Vanessa TillementAndreas Merdes
Aug 21, 2008·Cell Motility and the Cytoskeleton·Irina TikhonenkoMichael P Koonce
Feb 22, 2012·Current Biology : CB·Greg FitzHarris
Nov 13, 2009·Current Biology : CB·Sophie Dumont, Timothy J Mitchison
Oct 13, 2009·Current Biology : CB·David VannesteIsabelle Vernos
Jun 23, 2009·Current Biology : CB·Sophie Dumont, Timothy J Mitchison
May 19, 2007·Current Biology : CB·Ryoma OhiTimothy J Mitchison
Dec 15, 2010·Developmental Cell·Marvin E Tanenbaum, René H Medema
May 31, 2011·Journal of Neuroscience Methods·Joshua Chetta, Sameer B Shah
Oct 19, 2013·Developmental Cell·Elina VladimirouAndrew D McAinsh
May 6, 2016·Annual Review of Biophysics·Nenad Pavin, Iva M Tolić
Aug 24, 2013·Biology of Reproduction·Katie Howe, Greg FitzHarris
Nov 24, 2018·Biochemical Society Transactions·Claire T Friel, Julie P Welburn
Aug 14, 2020·Cellular and Molecular Life Sciences : CMLS·Anne NehligClara Nahmias

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