Kinesin-5 is not essential for mitotic spindle elongation in Dictyostelium

Cell Motility and the Cytoskeleton
Irina TikhonenkoMichael P Koonce

Abstract

The proper assembly and operation of the mitotic spindle is essential to ensure the accurate segregation of chromosomes and to position the cytokinetic furrow during cell division in eukaryotes. Not only are dynamic microtubules required but also the concerted actions of multiple motor proteins are necessary to effect spindle pole separation, chromosome alignment, chromatid segregation, and spindle elongation. Although a number of motor proteins are known to play a role in mitosis, there remains a limited understanding of their full range of functions and the details by which they interact with other spindle components. The kinesin-5 (BimC/Eg5) family of motors is largely considered essential to drive spindle pole separation during the initial and latter stages of mitosis. We have deleted the gene encoding the kinesin-5 member in Dictyostelium, (kif13), and find that, in sharp contrast with results found in vertebrate, fly, and yeast organisms, kif13(-) cells continue to grow at rates indistinguishable from wild type. Phenotype analysis reveals a slight increase in spindle elongation rates in the absence of Kif13. More importantly, there is a dramatic, premature separation of spindle halves in kif13(-) cells, suggesting a novel...Continue Reading

References

May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J Franke, R Kessin
Nov 1, 1993·The Journal of Cell Biology·M M HeckL S Goldstein
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y Y LiK Bloom
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D EshelI R Gibbons
Jan 18, 1996·Nature·A S KashinaJ M Scholey
Jul 24, 1997·Biochimica Et Biophysica Acta·A S KashinaJ M Scholey
Feb 7, 1998·Cell Motility and the Cytoskeleton·M P Koonce, D A Knecht
Feb 26, 2000·Molecular Biology of the Cell·M P Koonce, I Tikhonenko
Mar 21, 2000·Biochimica Et Biophysica Acta·E R Hildebrandt, M A Hoyt
Sep 19, 2000·Nature·D J SharpJ M Scholey
Sep 26, 2000·Cell Motility and the Cytoskeleton·M KimbleE L de Hostos
Jul 27, 2002·Annual Review of Cell and Developmental Biology·J Richard McIntoshRobert R West
Sep 4, 2003·Journal of Muscle Research and Cell Motility·Michael P Koonce, Alexey Khodjakov
Dec 3, 2003·BMC Genomics·Martin Kollmar, Gernot Glöckner
Jun 10, 2004·BMC Cell Biology·Andrea Hestermann, Ralph Gräf
Jul 9, 2004·Journal of Molecular Biology·Montserrat Samsó, Michael P Koonce
Sep 24, 2004·Current Biology : CB·Sharat Gadde, Rebecca Heald
Dec 8, 2004·The Journal of Cell Biology·David T MiyamotoTimothy J Mitchison
Apr 6, 2005·Developmental Cell·Stephan W Grill, Anthony A Hyman
Apr 28, 2005·Current Topics in Medicinal Chemistry·Gustave BergnesLisa Belmont
Apr 29, 2005·Molecular Biology of the Cell·Daniela A BritoMichael P Koonce
May 6, 2005·Nature·Lukas C KapiteinChristoph F Schmidt
Aug 9, 2005·Trends in Cell Biology·Harukata MikiNobutaka Hirokawa
Jan 13, 2006·Trends in Cell Biology·Alex MogilnerJonathan Scholey
Jan 13, 2006·Nature Chemical Biology·Michael A Lampson, Tarun M Kapoor
Feb 10, 2006·Journal of Molecular Biology·Xing MengMichael P Koonce
Mar 1, 2006·BMC Chemical Biology·Zoltan Maliga, Timothy J Mitchison
Apr 26, 2006·The Journal of Cell Biology·Lisa A CameronE D Salmon

❮ Previous
Next ❯

Citations

Nov 20, 2012·Cellular and Molecular Life Sciences : CMLS·Irina TikhonenkoMichael P Koonce
Jan 23, 2009·Molecular Biology of the Cell·Ingrid Brust-MascherJonathan M Scholey
Apr 24, 2008·BMC Cell Biology·Dilip K NagMichael P Koonce
Aug 25, 2015·Cellular and Molecular Life Sciences : CMLS·Irina TikhonenkoMichael P Koonce
Mar 1, 2016·Cell Reports·Alessandro De SimonePierre Gönczy
May 30, 2009·Cell Motility and the Cytoskeleton·Annette Müller-TaubenbergerGünther Gerisch
Mar 13, 2014·Biological Reviews of the Cambridge Philosophical Society·Danton H O'Day, Aldona Budniak
Nov 29, 2013·Cytoskeleton·Elizabeth CollinsPatricia Wadsworth
Aug 21, 2013·Gene·Edward J WojcikSunyoung Kim
Jun 22, 2013·European Journal of Cell Biology·Adrian J HarwoodAnnette Müller-Taubenberger
Jun 25, 2013·Current Biology : CB·Emma G Sturgill, Ryoma Ohi
Dec 15, 2010·Developmental Cell·Marvin E Tanenbaum, René H Medema
Jan 22, 2013·Biochemical and Biophysical Research Communications·Süleyman KösemAnnette Müller-Taubenberger
Dec 13, 2016·Biology·Jonathan M ScholeyIngrid Brust-Mascher
Apr 13, 2018·Cells·Michael P Koonce, Irina Tikhonenko
May 8, 2009·Molecular Biology of the Cell·Maureen WirschellWinfield S Sale
Sep 17, 2009·Biochemical Society Transactions·Kuniyoshi KasedaRobert A Cross
Jul 3, 2021·International Journal of Molecular Sciences·Himanshu PandeyLarisa Gheber

❮ Previous
Next ❯

Related Concepts

Related Feeds