DOI: 10.1101/476754Nov 22, 2018Paper

Kinesin-6 Klp9 plays motor-dependent and -independent roles in collaboration with Kinesin-5 Cut7 and the microtubule crosslinker Ase1 in fission yeast

BioRxiv : the Preprint Server for Biology
Takashi TodaKenya Furuta


Bipolar mitotic spindles play a critical part in accurate chromosome segregation. During late mitosis, spindle microtubules undergo drastic elongation towards the cell cortex in a process called anaphase B. Two kinesin motors, Kinesin-5 and Kinesin-6, are thought to generate outward forces to drive spindle elongation, and the microtubule crosslinker Ase1/PRC1 maintains structural integrity of antiparallel microtubules. However, how these three proteins orchestrate this process remains unknown. Here we explore the functional interplay among fission yeast Kinesin-5/Cut7, Kinesin-6/Klp9 and Ase1. Using total internal reflection fluorescence microscopy, we show that Klp9 is a processive plus end-directed motor. klp9ase1 is synthetically lethal. Surprisingly, this lethality is not ascribable to the defective motor activity of Klp9; instead, it is dependent upon an NLS and coiled coil domains within the non-motor region. We isolated a cut7 mutant (cut7-122) that displays temperature sensitivity only in the absence of Klp9. Interestingly, cut7-122 is impaired specifically in late mitotic stages. cut7-122klp9 double mutant cells exhibit additive defects in spindle elongation. Together, we propose that Klp9 plays dual roles during anaph...Continue Reading

Related Concepts

Microscopy, Fluorescence
Microtubule Proteins
Mitotic Spindle Apparatus
Schizosaccharomyces pombe
Anaphase-promoting complex
Cut7 protein, S pombe
Chromosome Segregation

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