Kinesin-6 regulates cell-size-dependent spindle elongation velocity to keep mitosis duration constant in fission yeast

ELife
Lara Katharina KrügerPhong T Tran

Abstract

The length of the mitotic spindle scales with cell size in a wide range of organisms during embryonic development. Interestingly, in C. elegans embryos, this goes along with temporal regulation: larger cells speed up spindle assembly and elongation. We demonstrate that, similarly in fission yeast, spindle length and spindle dynamics adjust to cell size, which allows to keep mitosis duration constant. Since prolongation of mitosis was shown to affect cell viability, this may resemble a mechanism to regulate mitosis duration. We further reveal how the velocity of spindle elongation is regulated: coupled to cell size, the amount of kinesin-6 Klp9 molecules increases, resulting in an acceleration of spindle elongation in anaphase B. In addition, the number of Klp9 binding sites to microtubules increases overproportionally to Klp9 molecules, suggesting that molecular crowding inversely correlates to cell size and might have an impact on spindle elongation velocity control.

References

Aug 1, 1990·The Journal of Cell Biology·C NislowJ R McIntosh
Aug 1, 1987·The Journal of Cell Biology·W M Saxton, J R McIntosh
Aug 1, 1986·The Journal of Cell Biology·W Z Cande, K McDonald
Feb 3, 1998·Molecular and Cellular Biology·J S Lanni, T Jacks
Sep 19, 2000·Nature·D J SharpJ M Scholey
Jun 26, 2002·The Journal of Cell Biology·Cristiana MollinariRobert L Margolis
May 26, 2004·Methods : a Companion to Methods in Enzymology·P T TranFred Chang
Jul 10, 2004·Current Biology : CB·Iva M Tolić-NørrelykkeFrancesco S Pavone
Aug 7, 2004·Current Biology : CB·Alexey KhodjakovFred Chang
Nov 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·I Brust-MascherJ M Scholey
Feb 4, 2005·Molecular Biology of the Cell·Isabelle LoïodiceP T Tran
May 6, 2005·Nature·Lukas C KapiteinChristoph F Schmidt
Aug 15, 2006·Nature Cell Biology·Vladimir VargaJonathon Howard
Jun 21, 2007·Current Biology : CB·Adam M SaundersWilliam M Saxton
Sep 15, 2007·Plant & Cell Physiology·Tomomi HayashiSeiichiro Hasezawa
May 13, 2008·Nature Cell Biology·Zhenye YangConly L Rieder
Aug 1, 2008·Molecular Biology of the Cell·Jérémie GaillardMarylin Vantard
Aug 6, 2008·The Journal of Cell Biology·Lukas C KapiteinErwin J G Peterman
Aug 23, 2008·Current Biology : CB·Martin WührTimothy J Mitchison
Jan 23, 2009·Molecular Biology of the Cell·Ingrid Brust-MascherJonathan M Scholey
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Michael Glotzer
Aug 18, 2009·Current Biology : CB·Yuki Hara, Akatsuki Kimura
Sep 14, 2010·Current Biology : CB·Yumi Uetake, Greenfield Sluder
Mar 8, 2011·Journal of Cell Science·Rachel Avunie-MasalaLarisa Gheber
Aug 19, 2011·Molecular Biology of the Cell·Felice D Kelly, Paul Nurse
Sep 6, 2011·Nature Cell Biology·Marcus BraunMarcel E Janson
Sep 23, 2011·Biochemical Society Transactions·Ingrid Brust-Mascher, Jonathan M Scholey
Oct 5, 2011·The Journal of Cell Biology·Nan MaPatricia Wadsworth
Dec 14, 2011·The Journal of Biological Chemistry·D Kenneth JamisonMichael R Diehl
Dec 16, 2011·Molecular Biology of the Cell·James D OrthTimothy J Mitchison
Mar 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Cécile LeducJonathon Howard
Nov 28, 2012·The Journal of Cell Biology·Shaila KotadiaAnne Royou
Dec 12, 2012·The Journal of Cell Biology·Neil J Ganem, David Pellman
Dec 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ken'ya FurutaHiroaki Kojima

❮ Previous
Next ❯

Citations

Oct 29, 2019·Cytoskeleton·Manuel Lera-Ramirez, François J Nédélec
Jun 6, 2020·Essays in Biochemistry·Lara K Krüger, Phong T Tran
Sep 19, 2019·Journal of Cell Science·Kruno VukušićIva M Tolić
Dec 7, 2020·Cytoskeleton·Katherine L Schutt, James B Moseley
Jul 2, 2021·Biophysical Journal·Dayna L MercadanteSarah D Olson
Jul 4, 2021·Scientific Reports·Masahito TanakaShigehiko Yumura

❮ Previous
Next ❯

Software Mentioned

Metamorph
Kaleidagraph

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.