PMID: 11319135Apr 25, 2001Paper

Kinesin light-chain KLC3 expression in testis is restricted to spermatids

Biology of Reproduction
Albert JuncoF A van der Hoorn

Abstract

Kinesins are tetrameric motor molecules, consisting of two kinesin heavy chains (KHCs) and two kinesin light chains (KLCs) that are involved in transport of cargo along microtubules. The function of the light chain may be in cargo binding and regulation of kinesin activity. In the mouse, two KLC genes, KLC1 and KLC2, had been identified. KLC1 plays a role in neuronal transport, and KLC2 appears to be more widely expressed. We report the cloning from a testicular cDNA expression library of a mammalian light chain, KLC3. The KLC3 gene is located in close proximity to the ERCC2 gene. KLC3 can be classified as a genuine light chain: it interacts in vitro with the KHC, the interaction is mediated by a conserved heptad repeat sequence, and it associates in vitro with microtubules. In mouse and rat testis, KLC3 protein expression is restricted to round and elongating spermatids, and KLC3 is present in sperm tails. In contrast, KLC1 and KLC2 can only be detected before meiosis in testis. Interestingly, the expression profiles of the three known KHCs and KLC3 differ significantly: Kif5a and Kif5b are not expressed after meiosis, and Kif5c is expressed at an extremely low level in spermatids but is not detectable in sperm tails. Our char...Continue Reading

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Citations

Sep 5, 2002·Molecular Reproduction and Development·Abraham L Kierszenbaum
Jun 16, 2012·The Cerebellum·Roberto Di FabioCarlo Casali
Feb 13, 2009·Reproduction, Fertility, and Development·Ifigenia OikonomopoulouPeter D Chantler
Feb 21, 2003·The Journal of Biological Chemistry·Bhupinder BhullarFrans A van der Hoorn
Oct 17, 2006·The Journal of Biological Chemistry·Carolyn FitzgeraldFrans A van der Hoorn
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Dec 26, 2018·Parasites & Vectors·Wanqi Hu, Zhijian Jake Tu

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