Kinetic analysis and modelling of enzymatic (R)-phenylacetylcarbinol batch biotransformation process

Journal of Biotechnology
Noppol LeksawasdiPeter L Rogers

Abstract

Initial rate and biotransformation studies were applied to refine and validate a mathematical model for enzymatic (R)-phenylacetylcarbinol (PAC) production from pyruvate and benzaldehyde using Candida utilis pyruvate decarboxylase (PDC). The rate of PAC formation was directly proportional to the enzyme activity level up to 5.0 U ml-1 carboligase. Michaelis-Menten kinetics were determined for the effect of pyruvate concentration on the reaction rate. The effect of benzaldehyde followed the sigmoidal shape of the Monod-Wyman-Changeux (MWC) model. The biotransformation model, which also included a term for PDC inactivation by benzaldehyde, was used to determine the overall rate constants for the formation of PAC, acetaldehyde, and acetoin. These values were determined from data for three batch biotransformations performed over a range of initial concentrations (viz. 50-150 mM benzaldehyde, 60-180 mM pyruvate, 1.1-3.4 U ml-1 enzyme activity). The finalized model was then used to predict a batch biotransformation profile at 120/100 mM initial pyruvate/benzaldehyde (initial enzyme activity 3.0 U ml-1). The simulated kinetics gave acceptable fitting (R2 = 0.9963) to the time courses of these latter experimental data for substrates pyr...Continue Reading

References

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Aug 2, 2003·Biotechnology Letters·Bettina RoschePeter L Rogers
Aug 2, 2003·Biotechnology Letters·Bettina RoschePeter L Rogers

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Citations

Nov 27, 2014·Applied Biochemistry and Biotechnology·Smita KandarSantosh B Noronha
Jul 13, 2018·Biotechnology Journal·Patrick C F BuchholzJürgen Pleiss
May 17, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Sarah T PoschenriederKathrin Castiglione

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