Kinetic analysis and substrate specificity of Escherichia coli dimethyl sulfoxide reductase

Microbiology
J L Simala-Grant, Joël H Weiner

Abstract

We have characterized the substrate specificity of dimethyl sulfoxide reductase (DmsABC) of Escherichia coli by determining Km and Kcat values for 22 different substrates. The enzyme has a very broad substrate specificity. The Km values varied 470-fold, while Kcat values varied only 20-fold, implicating Km as the major determinant of Kcat/Km values. Sulfoxides and pyridine N-oxide exhibited the lowest Km values, followed by aliphatic N-oxides. The Kcat values for these compounds also followed the same pattern. Substitution at the 2 or 3 position of the pyridine N-oxide ring had little effect on Km while substitution at the 4 position had a greater effect, and increased Km. Negatively charged substrates were poorly accepted. A few compounds that are not S- or N-oxides were also reduced by the enzyme. Most compounds reduced by DmsABC were not toxic to E. coli under anaerobic growth conditions, and E. coli was able to use many of these compounds anaerobically as terminal electron acceptors in the presence of glycerol. Anaerobic growth on sulfoxides is solely due to DmsABC expression. However, there appears to be another as yet unidentified terminal reductase capable of using pyridine N-oxides as terminal electron acceptors.

Citations

Nov 30, 2005·Biotechnology Letters·Olutosin DipeoluGill Stephens
Mar 20, 1999·Biochimica Et Biophysica Acta·W Reichenbecher, B Schink
Apr 19, 2013·Inorganic Chemistry·Golam MoulaSabyasachi Sarkar
Apr 20, 2001·Molecular Microbiology·I J OresnikR J Turner
Jun 27, 2009·Molecular Microbiology·Claudine BaraquetCécile Jourlin-Castelli
Feb 6, 2004·Biochimica Et Biophysica Acta·Paulina Geijer, Joel H Weiner
Oct 30, 2007·Biochimica Et Biophysica Acta·Richard A RotheryJoel H Weiner
Apr 26, 2011·Research in Microbiology·Francesca ItalianoLuigi R Ceci
Sep 10, 2004·The Journal of Biological Chemistry·Lodovica LoschiNatalie C J Strynadka
Oct 25, 2007·Biochemical Society Transactions·F Sargent
Jan 26, 2005·Biochemical Society Transactions·K HatzixanthisF Sargent
Mar 28, 2017·Protein Expression and Purification·Ewa NiedzialkowskaKrzysztof Lewiński
Dec 31, 2010·Inorganic Chemistry·Kajsa G V HaveliusMichael Haumann
Mar 14, 2021·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Navendu PaulSabyasachi Sarkar
Sep 1, 2010·EcoSal Plus·Tracy PalmerBen C Berks
Apr 1, 2007·EcoSal Plus·Victor W T Cheng, Joel H Weiner
Oct 3, 2003·Archives of Biochemistry and Biophysics·Shannon P Lubitz, Joel H Weiner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.