PMID: 9432753Aug 1, 1997Paper

Kinetic analysis of androstenedione 5 alpha-reductase in epithelium and stroma of human prostate

Steroids
H Weisser, M Krieg

Abstract

In the human prostate, various androgen-metabolizing enzymes are present. Among these enzymes, testosterone 5 alpha-reductase seems to be dominant. However, androstenedione is also a potential substrate of the prostatic 5 alpha-reductase. To address the question of to what extent the reduction of androstenedione to androstanedione occurs, the present study describes in detail the kinetic characteristics (Km and Vmax) and possible age-dependent alterations of this enzymatic step in epithelium and stroma of the human prostate. In normal prostate (NPR), the mean Km (nM) and Vmax (pmol/mg protein.h) were about twofold higher in stroma (Km, 211; Vmax, 130) than in epithelium (Km, 120; Vmax, 56), whereas in the benign prostatic hyperplasia (BPH), the mean Km (nM; mean +/- SEM) and Vmax (pmol/mg protein.h; mean +/- SEM) were about sixfold higher in stroma (Km, 668 +/- 121; Vmax, 415 +/- 73) than in epithelium (Km, 120 +/- 10; Vmax, 73 +/- 8). In BPH, those differences between epithelium and stroma were highly significant (p < 0.001). However, the efficiency ratios (Vmax/Km) of neither BPH nor NPR showed any significant differences between epithelium (NPR, 0.47; BPH, 0.62 +/- 0.06) and stroma (NPR, 0.70; BPH, 0.63 +/- 0.05). With respe...Continue Reading

References

Jan 1, 1986·Journal of Steroid Biochemistry·L MartiniM Motta
Jul 1, 1983·Journal of Steroid Biochemistry·P S RennieE E Dunstan-Adams
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·M KriegS Tunn
Jul 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·S TunnM Krieg
Jan 1, 1994·Annual Review of Biochemistry·D W Russell, J D Wilson
Aug 1, 1994·The Journal of Urology·R I SilverD W Russell
Dec 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·N BruchovskyP S Rennie

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Citations

Oct 21, 1998·The Journal of Steroid Biochemistry and Molecular Biology·H Weisser, M Krieg
Sep 9, 2010·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Xingsheng WangXiaobing Sang
Aug 5, 2006·Medicine and Science in Sports and Exercise·Gregory A BrownDouglas S King
Jul 25, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Sunhyae JangChang Deok Kim
Oct 21, 2004·Cancer·David G BostwickBarry Timms
Oct 12, 2012·Journal of Enzyme Inhibition and Medicinal Chemistry·Eugene BratoeffMarisa Cabeza
Feb 9, 2011·Journal of Enzyme Inhibition and Medicinal Chemistry·Marisa CabezaEugene Bratoeff
Jan 3, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sujeong KimEunsook Ma
Oct 9, 2019·International Journal of Environmental Research and Public Health·Young-Jin ChoiSi-Heung Sung

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