Kinetic analysis of cardiac transcriptome regulation during chronic high-fat diet in dogs

Physiological Genomics
Pierre Philip-CoudercP Rouet

Abstract

In the present study, we investigated, using custom dog cDNA arrays, the time course of transcriptional changes in the left ventricle of dogs fed a normal diet or a high-fat diet (HFD) for 9-24 wk. Array hybridizations were performed with complex probes representing mRNAs expressed in left ventricles from obese hypertensive and lean control dogs. We identified 63 differentially expressed genes, and expression of 17 of 20 randomly chosen genes was confirmed by real-time PCR. Transcripts were categorized into groups involved in metabolism, cell signaling, tissue remodeling, ionic regulation, cell proliferation, and protein synthesis. Hierarchical clustering indicated that the pattern of coregulated genes depends on duration of the HFD, suggesting that HFD-induced obesity hypertension is associated with continuous cardiac transcriptome adaptation despite stability of both body weight and blood pressure. GenMAPP analysis of the data pointed out the crucial importance of the ventricle TGF-beta pathway. Our results suggest that this system may be involved in molecular remodeling during HFD and in changes observed in the transcription profile, reflecting functional and morphological abnormalities that arise during prolonged HFD. These...Continue Reading

Associated Datasets

Jun 9, 2010·Pierre Philip-Couderc, Rouet Philippe

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Citations

Jan 22, 2009·Journal of Hypertension·Markus P SchlaichUNKNOWN Australian and New Zealand Obesity Society
May 9, 2006·Journal of the American Veterinary Medical Association·Kelly S Swanson
Apr 23, 2014·Physiology & Behavior·György Baffy, Joseph Loscalzo
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Sep 8, 2006·The Journal of Biological Chemistry·Matthieu LamantPhilippe Rouet
Apr 11, 2018·Frontiers in Physiology·Rashmi SupriyaParco M Siu

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