PMID: 3768412Oct 29, 1986Paper

Kinetic analysis of glycation as a tool for assessing the half-life of proteins

Biochimica Et Biophysica Acta
E Schleicher, O H Wieland

Abstract

Glycation of proteins, a common postribosomal modification, proceeds via Amadori rearrangement to yield a stable ketoamine linkage of glucose with the protein. Kinetic analysis of the reaction shows that the amount of glycation at steady state is proportional to the glucose concentration, to protein half-life and to the rate of glycation. Thus, when the rate of glycation is determined in vitro and the extent of glycation of a given protein isolated from euglycemic subjects is measured, the half-life may be calculated. As the in vivo situation may not be simulated accurately in vitro, the calculated values may be considered as approximation. When the calculated values were compared with values reported in the literature fairly good agreement was found except for hemoglobin. Studies on stability of glycated albumin show that ketoamine decreases by about 20% when incubated under physiological conditions for 20 days. The method described by us is especially valuable when turnover of proteins in normal and pathophysiological states are compared. The half-life of plasma low-density lipoprotein is longer in patients with hypothyroidism or a high plasma low-density lipoprotein level than in normal subjects. Extending our studies to tis...Continue Reading

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Citations

Jan 1, 1991·Diabetologia·Z MakitaS Nakagawa
Sep 15, 1988·Klinische Wochenschrift·E SchleicherK D Gerbitz
Dec 29, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·W KortlandtJ H Thijssen
Nov 30, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·N AhmedA J Furth
Mar 1, 1993·The International Journal of Biochemistry·M A Sheikh, D A Robb
Oct 9, 1999·Free Radical Biology & Medicine·R PamplonaG Barja
Dec 19, 2003·Advances in Renal Replacement Therapy·Thomas Henle, Toshio Miyata
May 23, 2014·Journal of the American Veterinary Medical Association·Kimberly M Pattullo, Beverly A Kidney
Jun 1, 1994·Kidney International·A G NerlichR Timpl
Aug 4, 2007·Kidney International. Supplement·E Schleicher, U Friess
Mar 8, 2016·Movement Disorders : Official Journal of the Movement Disorder Society·Hugo Vicente MirandaTiago Fleming Outeiro
May 28, 2011·Biotechnology and Bioengineering·Inn H YukJohn C Joly
Mar 20, 2010·Journal of Neurochemistry·Ruo-Li ChenJane Elizabeth Preston
Feb 27, 2010·The Journal of Pathology·Hugo Vicente Miranda, Tiago Fleming Outeiro
Feb 25, 1993·The American Journal of Cardiology·T J Lyons
Jan 20, 1998·Biochimica Et Biophysica Acta·Y KamisakiY Kishimoto
Jul 21, 2016·Glycoconjugate Journal·Naila RabbaniPaul J Thornalley
Jan 29, 2005·The Journal of Biological Chemistry·David R SellVincent M Monnier
Aug 3, 2018·International Journal of Legal Medicine·Aurora ValenzuelaBelén García-Villanova
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Nov 5, 2019·Frontiers in Neurology·Manuel Menendez-Gonzalez, Charles Gasparovic
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Apr 1, 2021·STAR Protocols·Stefania MarcottiBrian Marc Stramer
Sep 20, 1988·Journal of Molecular Biology·S TanakaE F Eikenberry

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