Kinetic analysis of glycine receptor currents in ventral cochlear nucleus

Journal of Neurophysiology
T P Harty, P B Manis

Abstract

Glycine plays an important role as an inhibitory neurotransmitter in the ventral cochlear nucleus. However, little is known about the kinetic behavior of glycine receptors. The present study examines the kinetics of the native inhibitory glycine receptors in neurons of the ventral cochlear nucleus, using outside-out patches from acutely dissociated cells and a fast flow system. Steps into 1 mM glycine revealed fast phases of desensitization with time constants of 13 and 129 ms, that together produced a 40% reduction in current from the peak response. Slower desensitization phases also were observed. After removal of glycine, currents deactivated with two time constants of 15 and 68 ms, and these rates were independent of the glycine concentration between 0.2 and 1 mM. Recovery from desensitization was slow relative to desensitization itself. These results demonstrate that glycine receptors can exhibit faster rates of desensitization and deactivation than previously reported.

References

Jun 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D A GodfreyF M Matschinsky
Oct 28, 1975·Pflügers Archiv : European journal of physiology·P R Adams
Mar 6, 1992·Brain Research·S KakehataN Akaike
Nov 27, 1992·Science·J D ClementsG L Westbrook
Oct 1, 1991·Journal of Neurophysiology·C A LewisD S Faber
Jan 1, 1992·Progress in Neurobiology·M Scuka, J W Mozrzymas
Jan 1, 1991·The Journal of Physiology·S Ito, E Cherubini
Nov 26, 1990·European Journal of Biochemistry·D LangoschH Betz
Aug 1, 1990·Journal of Neurophysiology·T C Yin, J C Chan
Jun 15, 1990·The Journal of Comparative Neurology·C G Benson, S J Potashner
Dec 1, 1989·Journal of Neurophysiology·N Akaike, M Kaneda
Oct 15, 1988·The Journal of Comparative Neurology·R J WentholdR A Altschuler
Feb 1, 1986·Brain Research Bulletin·A Frostholm, A Rotter
Feb 15, 1988·The Journal of Comparative Neurology·R E Wickesberg, D Oertel
May 1, 1986·International Journal of Bio-medical Computing·D Bertrand, C R Bader
Jan 1, 1968·Journal of Neurophysiology·R WermanM H Aprison
Jan 1, 1968·Experimental Brain Research·D R CurtisI H Johnston
Apr 24, 1984·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C B GundersenI Parker
Dec 1, 1980·Journal of Neuroscience Methods·D P Corey, A J Hudspeth
Apr 1, 1994·Biophysical Journal·J J Celentano, R K Wong

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Citations

Aug 17, 2002·The Journal of Pharmacology and Experimental Therapeutics·Gilles Martin, George Robert Siggins
Jun 2, 2005·Journal of Neuroscience Research·Mikiya AsakoRichard A Altschuler
Apr 22, 2015·Cell and Tissue Research·Eckhard FriaufMartin F Fuhr
Sep 19, 2002·Molecular Pharmacology·Pascal LegendreAntoine Triller
Feb 10, 2009·Nature Neuroscience·Veeramuthu BalakrishnanLaurence O Trussell
Jun 1, 2000·Hearing Research·K SatoR A Altschuler

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