Kinetic analysis of the binding of guanine nucleotide to bovine brain smg p25A

Biochemical and Biophysical Research Communications
I ShojiY Takai

Abstract

Bovine brain smg p25A, a guanine nucleotide-binding protein with a Mr of about 25,000, bound specifically GTP, guanosine 5'-(3-O-thio)triphosphate (GTP gamma S) and GDP. The initial velocities of the binding of GTP gamma S to GDP-bound smg p25A and the dissociation of GDP from this protein increased by decreasing Mg2+ concentrations or increasing NaCl concentrations. The initial velocity of the binding of GTP gamma S to GDP-free smg p25A was not affected by changing Mg2+ concentrations. These results indicate that the dissociation of GDP from smg p25A limits the binding of GTP to this protein, and suggest that there is a protein stimulating the dissociation of GDP from smg p25A and thereby stimulating the binding of GTP to this protein in mammalian tissues. In fact, the protein stimulating the dissociation of GDP, but not of GTP gamma S, from smg p25A was detected in bovine brain cytosol.

References

Sep 21, 1978·Biochimica Et Biophysica Acta·Y Kaziro
Jan 1, 1986·Annual Review of Neuroscience·L Stryer
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J B GibbsE M Scolnick
Jan 1, 1987·Annual Review of Biochemistry·M Barbacid
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N TouchotA Tavitian
Sep 1, 1987·Journal of Biochemistry·M HoshinoS Hattori
Feb 11, 1988·Nucleic Acids Research·A ZahraouiA Tavitian
May 1, 1986·The Biochemical Journal·V M Pain
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J SiekierkaS Ochoa

❮ Previous
Next ❯

Citations

Aug 14, 1992·Biochemical and Biophysical Research Communications·H SumimotoS Minakami
Jan 19, 1996·The Journal of Biological Chemistry·J Y PanM Wessling-Resnick
Jan 27, 1998·The Journal of Biological Chemistry·M Hosaka, T C Südhof
Oct 8, 1999·Biochemical and Biophysical Research Communications·J B ParkJ W Kim
Jul 15, 1992·Biochemical and Biophysical Research Communications·K AoyagiS Minakami

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.