PMID: 7525968Nov 4, 1994Paper

Kinetic analysis of the catalysis of strand transfer from internal regions of heteropolymeric RNA templates by human immunodeficiency virus reverse transcriptase

Journal of Molecular Biology
J J DeStefano

Abstract

The kinetic mechanism of HIV reverse transcriptase catalyzed strand transfer synthesis (i.e. switching of the primer to a new template) from internal regions of natural sequence RNA was investigated. The system consisted of a 142 nucleotide RNA template (donor), primed with a specific 20 nucleotide DNA oligonucleotide that was used to initiate synthesis. An RNA with homology to an internal region of the donor was used as acceptor template. Using 32P-labeled DNA oligonucleotide, the primer-extension products made from full-length synthesis on the donor (108 bases in length) or homologous transfer to and extension on the acceptor (155 bases) were monitored. Results indicated that the maximum efficiency of transfer (the ratio of transfer products to donor-directed+transfer products x 100) in this particular system was about 25% while the theoretical Vmax for the rate of appearance of transfer products at infinite acceptor concentration was about 20-fold lower than the measured rate for full-length donor-directed products. The Km for acceptor template in the transfer reaction was about 8 nM. Experiments using the above donor template hybridized to a specific DNA that has been shown to transfer to the acceptor indicated that RNase H...Continue Reading

References

Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D W Goodrich, P H Duesberg
Sep 1, 1990·Journal of Virology·G X Luo, J Taylor
Nov 30, 1990·Science·W S Hu, H M Temin
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H Xu, J D Boeke
Mar 1, 1989·Journal of Virology·F ClavelR Repaske
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·H M Temin

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Citations

Apr 8, 2000·Journal of Biomedical Science·J G Mikkelsen, F S Pedersen
Mar 30, 1999·Biochimica Et Biophysica Acta·B I KlasensB Berkhout
May 23, 2002·Current Opinion in Chemical Biology·Joshua A BittkerDavid R Liu
Sep 10, 2002·Nature Biotechnology·Joshua A BittkerDavid R Liu
Mar 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·David N LevyGeorge M Shaw
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·M Negroni, H Buc
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·H M Temin
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·P H Patel, B D Preston
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·M NegroniH Buc
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·L MoutouhD D Richman
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·R PatelE F Ullman
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·B CanardC C Richardson
Jul 25, 1995·Nucleic Acids Research·M A MartínezS Wain-Hobson
Sep 15, 2001·Nucleic Acids Research·A MoumenM Negroni
Sep 28, 2006·Nucleic Acids Research·Heather A BairdMatteo Negroni
Feb 14, 2006·Journal of Virology·Christian Lanciault, James J Champoux
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Adewunmi Onafuwa-Nuga, Alice Telesnitsky
Sep 3, 2004·Genetics·Daniel ShrinerJames I Mullins
Oct 14, 2011·Viruses·Krista Delviks-FrankenberryWei-Shau Hu
Jun 24, 2004·Progress in Nucleic Acid Research and Molecular Biology·K Andrew White, Peter D Nagy
Jul 19, 2011·Journal of Molecular Biology·Kun LuMichael F Summers
Feb 19, 2008·Virus Research·Vandana Purohit BasuRobert A Bambara
Dec 10, 2009·Phytotherapy Research : PTR·S K Kulkarni, Ashish Dhir
Feb 3, 2006·European Journal of Haematology·Vanessa TarerMarc Romana
Dec 13, 2007·The Journal of Biological Chemistry·Min SongRobert A Bambara

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