Kinetic analysis of the thermal stability of the photosynthetic reaction center from Rhodobacter sphaeroides

Biophysical Journal
Arwel V HughesM R Jones

Abstract

The temperature-induced denaturation of the photosynthetic reaction center from Rhodobacter sphaeroides has been studied through the changes that occur in the absorption spectrum of the bound chromophores on heating. At elevated temperatures, the characteristic absorbance bands of the bacteriochlorins bound to the polypeptides within the reaction center are lost, and are replaced by features typical of unbound bacteriochlorophyll and bacteriopheophytin. The kinetics of the spectral changes cannot be explained by a direct conversion from the functional to the denatured form of the protein, and require the presence of at least one intermediate. Possible mechanisms for the transformation via an intermediate are examined using a global analysis of the kinetic data, and the most likely mechanism is shown to involve a reversible transformation between the native state and an off-pathway intermediate, coupled to an irreversible transformation to the denatured state. The activation energies for the transformations between the three components are calculated from the effect of temperature on the individual rate constants, and the likely structural changes of the protein during the temperature-induced transformation are discussed.

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Citations

Jun 28, 2014·Biomolecules·Ernesto A Roman, F Luis González Flecha
Jan 12, 2008·Archives of Biochemistry and Biophysics·Diego I CattoniJosé M Argüello
Sep 5, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Marco MalferrariGiovanni Venturoli
Mar 18, 2009·Biochemical Society Transactions·Michael R Jones
Jul 4, 2019·Photosynthesis Research·David KaftanMichal Koblížek
Nov 7, 2019·Biochemistry. Biokhimii︠a︡·A A ZabelinA Ya Shkuropatov
Aug 23, 2007·Biochemistry·Paul K FyfeMichael R Jones
May 5, 2021·Biochemistry. Biokhimii︠a︡·Tatiana Yu Fufina, Lyudmila G Vasilieva
Aug 16, 2011·Methods : a Companion to Methods in Enzymology·Romina MancussoDa-Neng Wang

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