PMID: 9183007May 1, 1997Paper

Kinetic analysis of two closely related receptor-like protein-tyrosine-phosphatases, PTP alpha and PTP epsilon

European Journal of Biochemistry
K L LimCatherine J Pallen

Abstract

Among transmembrane protein-tyrosine-phosphatases, the membrane distal catalytic domain (D2) of protein-tyrosine-phosphatase alpha (PTP alpha) is unusual in having low but detectable activity in the absence of the membrane proximal catalytic domain (D1). To investigate the catalytic properties of PTP alpha D2 in association with D1, kinetic parameters of activity were established for PTP alpha D1D2 proteins containing an inactivating point mutation in D1 and/or D2. In this context, D2 activity was unchanged by the presence (N-terminal or C-terminal) or absence of inactive D1, and the presence or absence of inactive D2 affected the velocity but not the Km of D1 catalysis. While D1 appears to be the major catalytic contributor to PTP alpha activity, D2 possesses a significantly higher substrate-specific activity relative to wild-type D1D2 than the D2 domains of other protein-tyrosine-phosphatases. Also, PTP alpha D2 is an active phosphatase with comparable or better efficiency, on the basis of k(cat)/Km criteria, to some of the dual specificity phosphatases. Kinetic parameters of a closely related receptor-like protein-tyrosine-phosphatase, PTP epsilon, were determined. PTP epsilon D1 is the major, if not the only, catalytic moie...Continue Reading

References

Oct 4, 1991·Cell·W G Dunphy, A Kumagai
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R J MatthewsM L Thomas
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J SapJ Schlessinger
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M StreuliH Saito
Jan 5, 1995·Biochemical and Biophysical Research Communications·D H NgP Johnson
Nov 1, 1994·Trends in Biochemical Sciences·H Sun, N K Tonks
Apr 1, 1996·Current Opinion in Cell Biology·M Streuli

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Citations

Apr 2, 2005·Protein Expression and Purification·Maria A M SantosIgor Polikarpov
May 24, 2003·Biochimie·Günther H PetersNiels Peter H Møller
May 31, 2011·The Journal of Biological Chemistry·Haihong YeZhi-Cheng Xiao
May 14, 2005·Antioxidants & Redox Signaling·Annette Salmeen, David Barford
Mar 4, 2000·The EMBO Journal·X M ZhengD Shalloway
Apr 14, 2010·Molecular and Cellular Biology·Andrei M Vacaru, Jeroen den Hertog
Jul 27, 2000·Molecular and Cellular Biology·G JiangT Hunter
Jul 16, 2003·Molecular and Cellular Biology·Hila Toledano-KatchalskiAri Elson
Aug 14, 2013·PloS One·Dhaarmini RajshankarChristopher A McCulloch
May 7, 2015·Biochemical and Biophysical Research Communications·Yann FardiniTarik Issad
Apr 17, 1998·Critical Reviews in Biochemistry and Molecular Biology·Z Y Zhang
Dec 20, 2017·Nature Communications·Doreen BlüherUlla Bonas
Jun 29, 2001·The Journal of Biological Chemistry·H Gil-HennA Elson
May 16, 1998·The Journal of Biological Chemistry·V BhandariC J Pallen
Jun 19, 2001·American Journal of Physiology. Heart and Circulatory Physiology·L J ThompsonC Patterson
Mar 7, 2020·The Journal of Biological Chemistry·Yutao WenNunzio Bottini
Oct 6, 2018·Acta Crystallographica. Section D, Structural Biology·George T LountosDavid S Waugh
Jan 24, 1998·Biochemical and Biophysical Research Communications·R LammersA Ullrich
Oct 3, 1998·Trends in Biochemical Sciences·M J Wishart, J E Dixon

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