PMID: 9661814Jul 14, 1998Paper

Kinetic and affinity constants of epitope specific anti-carcinoembryonic antigen (CEA) monoclonal antibodies for CEA and engineered CEA domain constructs

Immunotechnology : an International Journal of Immunological Engineering
L J HeftaJ E Shively

Abstract

Carcinoembryonic antigen (CEA) is a human tumor antigen with the domain structure N-A1-B1-A2-B2-A3-B3, in which each domain is predicted to have an Ig-like fold and is known to bind epitope specific anti-CEA antibodies. To determine the affinity constants of several domain specific anti-CEA antibodies using purified recombinant or synthetic domains. We have determined the kinetic and affinity constants of several anti-CEA antibodies for CEA, CEA domains (A3-B3) expressed in HeLa cells, and a synthetic peptide corresponding to the A3 domain using a BIAcore biosensor. There was no difference in affinity for CEA among a murine (mT84.66), a mouse/human chimeric form (cT84.66) or a disulfide deleted version (delta SScT84.66) of this antibody. There was less than a five-fold drop in affinity of murine T84.66 for the A3-B3 domain expressed in HeLa cells compared to CEA. The synthetic A3 domain had an affinity constant for mT84.66 which was ten-fold less than for CEA. The affinity constants for CEA with several other anti-CEA monoclonal antibodies, including three antibodies which have almost identical CDR sequences (CEA.281, CEA.11 and CEM231) were also determined. CEM231 which had a two-fold higher affinity constant for CEA than eith...Continue Reading

Citations

Dec 28, 1999·Journal of Molecular Recognition : JMR·D G Myszka
Dec 13, 2005·Annals of Biomedical Engineering·Gregory Z FerlJoseph J DiStefano
Nov 28, 2012·Journal of Pharmacokinetics and Pharmacodynamics·Lubna Abuqayyas, Joseph P Balthasar
Mar 31, 2001·Cancer Biotherapy & Radiopharmaceuticals·L E WilliamsJ Y Wong
Sep 6, 2008·Cancer Biotherapy & Radiopharmaceuticals·James R BadingSven E Strand
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·C VaqueroR Fischer
Aug 6, 2005·Scandinavian Journal of Clinical and Laboratory Investigation·J Bjerner
Jul 21, 2004·Journal of Immunological Methods·Anthony WarfordJohn McCafferty
Jun 19, 2014·Materials·Niina J Ronkainen, Stanley L Okon
Aug 15, 2002·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·R Gambari
Jun 19, 2014·Molecular and Clinical Oncology·Yunlong DingShuangyi Ren
Jul 5, 2006·Molecular Cancer Therapeutics·Gregory Z FerlJoseph J DiStefano

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