Kinetic and equilibrium studies on the polyazamacrocycle neotetren: metal-complex formation and DNA interaction

Dalton Transactions : an International Journal of Inorganic Chemistry
Tarita BiverBarbara Valtancoli

Abstract

The macrocyclic polyamine 2,5,8,11,14-pentaaza[15]-[15](2,9)[1,10]phenanthrolinophane (neotetren) is studied in its ability to coordinate Cu(ii) even at very low pH values and to interact, as a metal complex, with DNA. The kinetics and equilibria for 1 : 1 and 2 : 1 metal-ligand complexes formation are studied by the stopped-flow method and UV spectrophotometry. Differently protonated complexes are formed, with rate constants much lower than that of water exchange at copper(II) and other Cu(II)/amine systems, this behaviour being ascribed to ring effects and intra-molecular hydrogen bonds. Concerning the DNA/copper(II)-neotetren complexes interaction, analysis of data suggests an intercalative mode of binding. The kinetic results for both DNA/CuL and DNA/Cu(2)L systems agree with the sequence D + S <-->D,S <-->DS where the metal complexes (D) react with the DNA sites (S) leading to fast formation of an externally bound form (D,S) which is converted into an intercalated complex (DS). A very slow process is also detected and ascribed to a conformational change in the polynucleotide secondary structure where the metal centre plays a crucial role. Chromatographic experiments demonstrate that both the investigated Cu(II)/L complexes...Continue Reading

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Citations

Jan 15, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Md Abdus Subhan, Jong-Ha Choi
Aug 19, 2014·Physical Chemistry Chemical Physics : PCCP·A BiancardiM Venturini
Sep 15, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Carla BazzicalupiMarcella Venturini
May 16, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Maria R BecciaMarcella Venturini
Jan 21, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Rebeca RuizMarta González-Alvarez
Feb 21, 2013·Physical Chemistry Chemical Physics : PCCP·Alessandro BiancardiBenedetta Mennucci
May 23, 2012·Chemistry, an Asian Journal·Maria Rosa BecciaRafael Prado Gotor
Nov 5, 2016·Journal of Inorganic Biochemistry·Sabriye AydinogluClaudia Pellerito
Sep 14, 2007·Chemistry : a European Journal·Carla BazzicalupiMarcella Venturini
Aug 22, 2018·Inorganic Chemistry·Álvaro Martínez-CamarenaEnrique García-España

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