Kinetic and functional analysis of transient, persistent and resurgent sodium currents in rat cerebellar granule cells in situ: an electrophysiological and modelling study

The Journal of Physiology
Jacopo MagistrettiEgidio D'Angelo

Abstract

Cerebellar neurones show complex and differentiated mechanisms of action potential generation that have been proposed to depend on peculiar properties of their voltage-dependent Na+ currents. In this study we analysed voltage-dependent Na(+) currents of rat cerebellar granule cells (GCs) by performing whole-cell, patch-clamp experiments in acute rat cerebellar slices. A transient Na+ current (I(NaT)) was always present and had the properties of a typical fast-activating/inactivating Na+ current. In addition to I(NaT), robust persistent (I(NaP)) and resurgent (I(NaR)) Na+ currents were observed. I(NaP) peaked at approximately -40 mV, showed half-maximal activation at approximately -55 mV, and its maximal amplitude was about 1.5% of that of I(NaT). I(NaR) was elicited by repolarizing pulses applied following step depolarizations able to activate/inactivate I(NaT), and showed voltage- and time-dependent activation and voltage-dependent decay kinetics. The conductance underlying I(NaR) showed a bell-shaped voltage dependence, with peak at -35 mV. A significant correlation was found between GC I(NaR) and I(NaT) peak amplitudes; however, GCs expressing I(NaT) of similar size showed marked variability in terms of I(NaR) amplitude, and...Continue Reading

References

Mar 1, 1988·The Journal of General Physiology·P SahP W Gage
Mar 1, 1994·Journal of Neurophysiology·T R CumminsG G Haddad
Aug 15, 1997·Neural Computation·M L Hines, N T Carnevale
Oct 9, 1999·Journal of Computational Neuroscience·M C Vanier, J M Bower
Dec 11, 1999·Brain Research·F Pan, K G Beam
Nov 27, 2002·The Journal of General Physiology·Jacopo Magistretti, Angel Alonso
Jul 29, 2003·The Cerebellum·Kristin L Schaller, John H Caldwell
Nov 25, 2003·The Journal of Physiology·Philippe IsopePhilippe Ascher
Apr 23, 2004·Nature·Paul ChaddertonMichael Häusser
Jun 24, 2004·Journal of Neurophysiology·Fatemeh S AfshariIndira M Raman
Apr 7, 2005·FEBS Letters·Theodore R CumminsStephen G Waxman

❮ Previous
Next ❯

Citations

Sep 29, 2011·Cellular and Molecular Life Sciences : CMLS·Mitchell Goldfarb
Oct 5, 2010·Acta Physiologica·R K Pradhan, V S Chakravarthy
Dec 17, 2008·Journal of Neurophysiology·Shyam DiwakarEgidio D'Angelo
Dec 30, 2009·The Journal of Clinical Investigation·Stephen C Cannon, Bruce P Bean
Feb 15, 2012·Neural Systems & Circuits·Fabio M Simões de Souza, Erik De Schutter
Nov 19, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jun Hee KimHenrique von Gersdorff
May 18, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eric J SchwartzStéphane Dieudonné
Jun 4, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lorin S MilescuJeffrey C Smith
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jason S Bant, Indira M Raman
Dec 3, 2008·The Journal of Physiology·David BurkeMatthew C Kiernan
Feb 23, 2010·The Journal of Physiology·Nancy OsorioPatrick Delmas
Apr 19, 2015·Molecular Pain·Melissa A TadrosRobert J Callister
Aug 31, 2014·The Journal of Physiology·Amanda H Lewis, Indira M Raman
Nov 4, 2008·Trends in Neurosciences·Egidio D'Angelo, Chris I De Zeeuw
Jul 12, 2008·Bio Systems·Richard R CarrilloEgidio D'Angelo
Dec 15, 2010·Brain Research·Mantas GabrielaitisAidas Alaburda
Jun 21, 2011·Life Sciences·Jader S CruzFernando A Oliveira
Dec 27, 2008·Neuron·Hai Huang, Laurence O Trussell
Sep 6, 2014·Frontiers in Cellular Neuroscience·Sathyaa SubramaniyamEgidio D'Angelo
May 24, 2016·Journal of Therapeutic Ultrasound·Elisabetta Sassaroli, Natalia Vykhodtseva
Sep 27, 2013·PLoS Computational Biology·Jorge F MejiasAndré Longtin
Apr 8, 2015·PloS One·Jonathan MapelliAlbertino Bigiani
Mar 22, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Clare HowarthDavid Attwell
Dec 2, 2017·The Journal of Physiology·Hui HongJason Tait Sanchez
Oct 12, 2018·The Journal of General Physiology·Tadashi YamanishiJeffrey C Smith
May 22, 2007·Nature Reviews. Neuroscience·Bruce P Bean
Dec 13, 2006·The Journal of Physiology·Anthony M RushStephen G Waxman
Jun 22, 2019·PLoS Computational Biology·Sharmila VenugopalScott H Chandler
Jul 16, 2020·Journal of Neurophysiology·Frances L Meredith, Katherine J Rennie
Mar 20, 2018·Brain : a Journal of Neurology·Floriana FruscioneFabio Benfenati

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.