Kinetic and inhibition studies on reduction of diphenyl sulfoxide by guinea pig liver aldehyde oxidase

Archives of Biochemistry and Biophysics
S Yoshihara, K Tatsumi

Abstract

To characterize the properties of diphenyl sulfoxide (DPSO) as a new type of electron acceptor for guinea pig liver aldehyde oxidase (AO), we compared the kinetics of the reductions of DPSO and other classical electron acceptors such as O2 and ferricyanide. The double-reciprocal plot of the 2-hydroxypyrimidine (2-OH PM)-linked DPSO reduction with the highly purified enzyme was biphasic. Similar biphasic plots were obtained with the reductions of other electron acceptors. Only the lower Km value, which was obtained by extrapolation of the plot at lower concentrations of 2-OH PM, was identical with that shown by the freshly prepared crude enzyme. DPSO as well as menadione progressively inhibited the reductions of O2 and ferricyanide with time. Menadione inhibited the DPSO reduction noncompetitively with respect to 2-OH PM and competitively with respect to DPSO, while its mode of inhibition of ferricyanide reduction was uncompetitive for either the electron donor or the acceptor. On the other hand, DPSO showed an uncompetitive and a noncompetitive inhibition of ferricyanide reduction with respect to 2-OH PM and ferricyanide, respectively. These results may indicate that DPSO interacts with the enzyme at the same site as menadione,...Continue Reading

References

Oct 1, 1985·Archives of Biochemistry and Biophysics·S Yoshihara, K Tatsumi
Mar 30, 1982·Biochemistry·M J BarberL M Siegel
Sep 14, 1983·Biochimica Et Biophysica Acta·K TatsumiH Yamada
Oct 31, 1983·Biochemical and Biophysical Research Communications·P E GormleyR Cysyk

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Citations

Feb 16, 2002·European Journal of Biochemistry·Michael ColemanJanet Hemingway
Jun 30, 1987·Toxicology and Applied Pharmacology·J M Fraser, L S Kaminsky
Apr 27, 2017·Bioconjugate Chemistry·Kuan HuZigang Li
Nov 2, 2018·European Journal of Drug Metabolism and Pharmacokinetics·Farnaz Deris-AbdolahpourMohammad-Reza Rashidi
Jun 27, 2003·Journal of Clinical Psychopharmacology·Christine BeedhamR Scott Obach
Nov 7, 2002·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·B G LakeJ A Scatina
Feb 22, 2007·Chemical Research in Toxicology·Charles G KnutsonLawrence J Marnett
May 26, 1995·Biochemical Pharmacology·S C Lee, A G Renwick

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