Kinetic and molecular identification of sodium-dependent glucose transporter in normal rat cholangiocytes

The American Journal of Physiology
K N LazaridisN F LaRusso

Abstract

While previous work has demonstrated that monosaccharides can be absorbed from bile, studies of sugar transport by the biliary, epithelia (i.e., cholangiocytes) are lacking. Using a novel model of polarized rat cholangiocytes in primary culture, designated normal rat cholangiocytes (NRC), we examined directly the uptake and transcellular transport of a nonmetabolizable monosaccharide, methyl alpha-D-glucopyranoside (AMG). When the apical or basolateral domain of cholangiocytes was exposed to radiolabeled AMG or sucrose (control), only apical absorption of AMG was evident. This apical uptake was time dependent, saturable, and significantly inhibited (> or = 90%) by removal of Na+ or in the presence of phlorizin (0.1 mM), a competitive inhibitor of the Na(+)-glucose cotransporter. The transcellular flux of AMG was also polar (i.e., apical to basolateral). Reverse transcriptase-polymerase chain reaction (RT-PCR) revealed the presence of the transcript for the specific Na(+)-glucose cotransporter SGLT1 in NRC and in freshly isolated cholangiocytes but not in purified hepatocytes; in contrast, the transcript for SGLT2 was absent in all liver samples. In situ RT-PCR on frozen sections of normal rat liver showed that SGLT1 was express...Continue Reading

Citations

Aug 16, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Albert MennoneJames L Boyer
Aug 24, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·N KannoG Alpini
Apr 30, 2011·Physiological Reviews·Ernest M WrightBruce A Hirayama
Feb 24, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·A MennoneJ L Boyer
Feb 8, 2002·World Journal of Gastroenterology : WJG·Pamela S TietzNicholas F LaRusso
Jun 6, 2008·American Journal of Physiology. Cell Physiology·Daniela BalenIvan Sabolic
Mar 1, 2016·Biophysics Reviews·Archana M Navale, Archana N Paranjape
Aug 15, 2002·American Journal of Physiology. Cell Physiology·Anatoly I MasyukNicholas F LaRusso
Apr 1, 2004·American Journal of Physiology. Endocrinology and Metabolism·Raul C CamachoDavid H Wasserman
Apr 5, 2003·American Journal of Physiology. Cell Physiology·Ai-Yu GongNicholas F LaRusso
Oct 12, 2014·Pflügers Archiv : European journal of physiology·Ivana VrhovacHermann Koepsell
Sep 23, 2021·Pflügers Archiv : European journal of physiology·Shari WiegreffeDominik Wiemuth

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