PMID: 6967404May 1, 1980Paper

Kinetic and steady-state investigations of solute accumulation in bacterial membranes by continuously monitoring the radioactivity in the effluent of flow-dialysis experiments

European Journal of Biochemistry
K J Hellingwerf, W N Konings

Abstract

The flow-dialysis technique for studies of solute accumulation by membrane preparations has been made more suitable for routine measurements by recording continuously the radioactivity in the effluent of a flow-dialysis vessel with a homogeneous flow-monitoring device for beta-emitters. This modification not only decreases the time and cost of a flow-dialysis experiment but also allows the investigator to react directly on the outcome of his experiments. Analysis of the kinetics of this automated flow-dialysis system shows that this technique can also be used for the determination of the rate of uptake of solutes into bacterial membranes. This has been confirmed in Rhodopseudomonas sphaeroides by comparing the results of Rb+ and inorganic phosphate uptake studies performed by automated flow-dialysis and by the conventional filtration procedure. This application has the limitation that solute uptake has to proceed linearly for a period of about five times the half-time of the response of the flow-dialysis system. The two described applications make automated flow-dialysis very well-suited for experiments on the bioenergetics and regulation of solute uptake into bacterial membranes. Both driving force and rate of solute uptake ca...Continue Reading

References

Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S RamosH R Kaback
Feb 7, 1977·Biochimica Et Biophysica Acta·C S HuangC P Lee
May 2, 1977·Biochimica Et Biophysica Acta·I Friedberg
Sep 11, 1979·Biochimica Et Biophysica Acta·K J HellingwerfH V Westerhoff
Feb 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·A AzziC P Lee
Aug 13, 1971·Biochimica Et Biophysica Acta·N Medveczky, H Rosenberg

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Citations

Feb 1, 1989·Journal of Biochemical and Biophysical Methods·H V WesterhoffK J Hellingwerf
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A J DriessenW N Konings
Jun 1, 1981·European Journal of Biochemistry·M Michels, E P Bakker
Jul 15, 1983·European Journal of Biochemistry·J Bergsma, W N Konings
Nov 15, 1985·European Journal of Biochemistry·M G ElferinkW N Konings
Jan 1, 1989·Critical Reviews in Microbiology·W N KoningsA J Driessen
Nov 2, 1993·Biochimica Et Biophysica Acta·B Poolman, W N Konings
May 1, 1981·European Journal of Biochemistry·K NicolayW N Konings
Oct 1, 1982·European Journal of Biochemistry·H HaakerC Veeger
Jun 1, 1982·Journal of Bacteriology·K J HellingwerfW N Konings
Nov 1, 1987·Journal of Bacteriology·A J DriessenW N Konings
Feb 1, 1987·Applied and Environmental Microbiology·J HugenholtzH Veldkamp
Jan 1, 1986·Applied and Environmental Microbiology·A van Boven, W N Konings
Nov 15, 1984·Archives of Biochemistry and Biophysics·B A MelandriL Packer

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