PMID: 11330348May 2, 2001Paper

Kinetic and structural properties of two isoforms of trypsin isolated from the viscera of Japanese anchovy, Engraulis japonicus

Journal of Protein Chemistry
M N Ahsan, S Watabe

Abstract

Two isoforms of anchovy trypsin (aT-I and aT-II) were purified from the visceral extracts by (NH4)2SO4 fractionation followed by affinity chromatography, gel filtration, and ion-exchange chromatography. The homogeneity of the purified preparation was evidenced by both native- and SDS-PAGE, and further by gelatin zymography. Identities of aT-I and aT-II as trypsins were established by N-terminal amino acid sequencing, which matched exactly to the corresponding stretches of their respective amino acid sequences obtained by molecular cloning [Ahsan et al. (2000), Marine Biotechnol., in press]. Both isoforms were completely inhibited by serine protease inhibitors as well as by specific trypsin inhibitors. The purified anchovy trypsins showed considerably higher catalytic efficiencies (kcat/Km) than bovine trypsin as measured toward benzoyl-arginine p-nitroanilide (BAPA) and benzoyl-arginine ethyl ester (BAEE) at 25 degrees C; in particular, aT-II was 35 times more efficient than its mammalian counterpart against BAPA. This was due mainly to a dramatic decrease of Km values for anchovy trypsins, which are indicative of an evolutionary response toward increased substrate binding at suboptimal temperatures in the marine environment.

Citations

Sep 7, 2014·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Erick PereraJuan Miguel Mancera
Mar 21, 2018·3 Biotech·Kristal Jesús-de la CruzÁngela Ávila-Fernández
Jun 28, 2006·Biological Chemistry·Tadaaki KishiEleftherios P Diamandis
Sep 11, 2007·Biological & Pharmaceutical Bulletin·Eiko ToyotaKazutaka Tanizawa
Feb 9, 2012·Journal of Amino Acids·Tomoyoshi FuchiseByung-Soo Chun
Jun 8, 2012·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Cenk Daglioglu, Figen Zihnioglu
Apr 18, 2016·International Journal of Biological Macromolecules·Ahmad HomaeiReyhaneh Sariri
Feb 7, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·I KurtovicB K Simpson
Nov 17, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Bjarki StefanssonJón B Bjarnason

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
E SiigurJ Siigur
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Francisco Javier Castillo-YáñezMaría de Los Angeles Navarrete-Del Toro
© 2021 Meta ULC. All rights reserved