Kinetic and thermodynamic resolution of the interactions between sulfite and the pentahaem cytochrome NrfA from Escherichia coli

The Biochemical Journal
Gemma KempJulea N Butt

Abstract

NrfA is a pentahaem cytochrome present in a wide-range of γ-, δ- and ε-proteobacteria. Its nitrite and nitric oxide reductase activities have been studied extensively and contribute to respiratory nitrite ammonification and nitric oxide detoxification respectively. Sulfite is a third substrate for NrfA that may be encountered in the micro-oxic environments where nrfA is expressed. Consequently, we have performed quantitative kinetic and thermodynamic studies of the interactions between sulfite and Escherichia coli NrfA to provide a biochemical framework from which to consider their possible cellular consequences. A combination of voltammetric, spectroscopic and crystallographic analyses define dissociation constants for sulfite binding to NrfA in oxidized (~54 μM), semi-reduced (~145 μM) and reduced (~180 μM) states that are comparable with each other, and the Km (~70 μM) for sulfite reduction at pH 7. Under comparable conditions Km values of ~22 and ~300 μM describe nitrite and nitric oxide reduction respectively, whereas the affinities of nitrate and thiocyanate for NrfA fall more than 50-fold on enzyme reduction. These results are discussed in terms of the nature of sulfite co-ordination within the active site of NrfA and th...Continue Reading

References

May 1, 1984·Journal of General Microbiology·N R Pope, J A Cole
Jul 25, 1996·Biochemical and Biophysical Research Communications·I C PereiraM Teixeira
Nov 21, 1998·Journal of Leukocyte Biology·H MitsuhashiT Naruse
Aug 10, 1999·Nature·O EinsleP M Kroneck
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·A G Leslie
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jun 1, 2000·Journal of Inorganic Biochemistry·P StachP M Kroneck
Apr 19, 2002·The Journal of Biological Chemistry·Susannah R PoockDavid J Richardson
Aug 8, 2002·FEMS Microbiology Reviews·Jörg Simon
Sep 26, 2002·Journal of the American Chemical Society·Oliver EinsleFrank Neese
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Serge X CohenAnastassis Perrakis
Jan 26, 2005·Biochemical Society Transactions·B BurlatD J Richardson
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Philip Evans
Apr 25, 2007·Nucleic Acids Research·Ian W DavisDavid C Richardson
Jan 19, 2008·Biochemistry·Peer LukatOliver Einsle
Feb 5, 2008·The Journal of Biological Chemistry·Jessica H van WonderenJulea N Butt
Apr 25, 2008·Methods in Enzymology·Thomas A ClarkeDavid J Richardson
Sep 27, 2008·Acta Pharmacologica Sinica·Hong-fang JinJun-bao Du

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Citations

Jan 28, 2012·Acta Crystallographica. Section D, Biological Crystallography·Anton A TrofimovVladimir O Popov
Nov 20, 2012·Biochemistry. Biokhimii︠a︡·T V TikhonovaV O Popov
Jun 19, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Rose-Marie A S DoyleJulea N Butt
Jan 30, 2016·BMC Microbiology·Tamara M KhlebodarovaVitaly A Likhoshvai
Jan 27, 2011·Biochemical Society Transactions·Andrew J GatesJulea N Butt
Feb 3, 2015·Nature·Bianca HermannOliver Einsle
Apr 4, 2014·Chemical Reviews·Luisa B Maia, José J G Moura
May 7, 2015·Acta Crystallographica. Section D, Biological Crystallography·A A TrofimovV O Popov
Mar 14, 2021·The Science of the Total Environment·Bo WuShanquan Wang
Feb 7, 2015·Journal of the American Chemical Society·Colin W J LockwoodJulea N Butt

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