Kinetic approach for the enzymatic determination of levodopa and carbidopa assisted by multivariate curve resolution-alternating least squares

Analytica Chimica Acta
Marcos GrünhutBeatriz S Fernández Band

Abstract

A combination of kinetic spectroscopic monitoring and multivariate curve resolution-alternating least squares (MCR-ALS) was proposed for the enzymatic determination of levodopa (LVD) and carbidopa (CBD) in pharmaceuticals. The enzymatic reaction process was carried out in a reverse stopped-flow injection system and monitored by UV-vis spectroscopy. The spectra (292-600 nm) were recorded throughout the reaction and were analyzed by multivariate curve resolution-alternating least squares. A small calibration matrix containing nine mixtures was used in the model construction. Additionally, to evaluate the prediction ability of the model, a set with six validation mixtures was used. The lack of fit obtained was 4.3%, the explained variance 99.8% and the overall prediction error 5.5%. Tablets of commercial samples were analyzed and the results were validated by pharmacopeia method (high performance liquid chromatography). No significant differences were found (alpha=0.05) between the reference values and the ones obtained with the proposed method. It is important to note that a unique chemometric model made it possible to determine both analytes simultaneously.

References

Apr 6, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Morvarid KarimiJoel S Perlmutter
Mar 15, 2007·Talanta·Marcos GrünhutBeatriz S Fernández Band

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Citations

May 12, 2012·Journal of Analytical Methods in Chemistry·Supaporn Kradtap Hartwell, Kate Grudpan
May 14, 2021·Journal of Nanoscience and Nanotechnology·Z Z WangH P Zhang

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