PMID: 9635250Jun 23, 1998Paper

Kinetic characterization of bovine lung low-molecular-weight protein tyrosine phosphatase

Experimental Lung Research
M A BuzalafH Aoyama

Abstract

Protein tyrosine phosphatase is an important class of enzymes that plays an essential role in the cellular proliferation, differentiation, and oncogenesis. In this paper we report characterization of a low-molecular-weight protein tyrosine phosphatase purified from bovine lung. The enzyme activity was essentially independent of metal ions and sensitive to sulfhydryl reagents. Both vanadate and inorganic phosphate are competitive inhibitors, with Ki values of 0.38 microM and 0.28 mM, respectively. Besides p-nitrophenyl phosphate, the enzyme was also able to efficiently hydrolyze tyrosine phosphate, beta-naphthyl phosphate, and flavine mononucleotide.

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Citations

Apr 10, 2014·BMC Research Notes·Tiago Henrique RombolaJoão Martins Pizauro
Jun 29, 2001·Experimental Eye Research·I O UmedaH Nishigori
Oct 25, 2006·Journal of Enzyme Inhibition and Medicinal Chemistry·Márcio A MirandaHiroshi Aoyama
Mar 7, 2015·Journal of the American Society of Nephrology : JASN·Nima AbbasianAlan Bevington
Feb 3, 2016·The Journal of Microbiology·Vanessa Sayuri SatoJoão Martins Pizauro Junior

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