Kinetic characterization of P-type membrane ATPase from Streptococcus mutans

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Prislaine P MagalhãesPietro Ciancaglini

Abstract

The proton translocating membrane ATPase of oral streptococci has been implicated in cytoplasmatic pH regulation, acidurance and cariogenicity. Studies have confirmed that Streptococcus mutans is the most frequently detected species in dental caries. A P-type ATPase that can act together with F(1)F(o)-ATPase in S. mutans membrane has been recently described. The main objective of this work is to characterize the kinetic of ATP hydrolysis of this P-type ATPase. The optimum pH for ATP hydrolysis is around 6.0. The dependence of P-type ATPase activity on ATP concentration reveals high (K(0.5)=0.27 mM) and low (K(0.5)=3.31 mM) affinity sites for ATP, exhibiting positive cooperativity and a specific activity of about 74 U/mg. Equimolar concentrations of ATP and magnesium ions display a behavior similar to that described for ATP concentration in Mg(2+) saturating condition (high affinity site, K(0.5)=0.10 mM, and low affinity site, K(0.5)=2.12 mM), exhibiting positive cooperativity and a specific activity of about 68 U/mg. Sodium, potassium, ammonium, calcium and magnesium ions stimulate the enzyme, showing a single saturation curve, all exhibiting positive cooperativities, whereas inhibition of ATPase activity is observed for zinc i...Continue Reading

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Citations

Sep 3, 2011·The Journal of Microbiology·Fatih Saban BerisAli Osman Belduz
Jul 24, 2010·Photomedicine and Laser Surgery·M BoleanPietro Ciancaglini
May 31, 2008·Biotechnology and Bioengineering·Adrienne C GreeneGeorge D Bachand
Nov 20, 2013·Journal of Digestive Diseases·Wen Ming WuLi Hua Peng
Apr 27, 2016·Molecular Oral Microbiology·J L BakerRobert G Quivey
Jan 17, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Geraldo ThedeiPietro Ciancaglini
May 12, 2017·Archives of Oral Biology·M A Bojanich, R O Calderón
Jul 29, 2020·Critical Reviews in Microbiology·Karthi ShanmugamPrasanna Neelakantan

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Quaternary Ammonium Compounds
Calcium
Catalysis
Edetic Acid, Sodium Salt
H(+)-Transporting ATP Synthase, Acyl-Phosphate-Linked
Hydrogen-Ion Concentration
Hydrolysis
Magnesium
Cell Surface Proteins

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