Kinetic characterization of yeast pyruvate carboxylase isozyme pyc1

Biochemistry
J P BransonP V Attwood

Abstract

Yeast (Saccharomyces cerevisiae) is unusual in being the only organism thus far identified as having two genes for pyruvate carboxylase. The expression of the two isozymes Pyc1 and Pyc2 appears to be differentially regulated, and since both are expressed cytoplasmically, this suggests that they have different properties. To the present, little has been done to characterize these isozymes, and almost all of the published kinetic information on yeast pyruvate carboxylase comes from measurements of enzyme prepared from bakers' yeast which is likely to be a mixture of both isozymes. Here we have measured basic kinetic parameters for Pyc1 and found that the K(a) of this isozyme for acetyl CoA is in the order of 8-10-fold higher than previously recorded, suggesting that Pyc1 and Pyc2 may be differentially regulated by this effector. Pyc1 is highly dependent on the presence of acetyl CoA for activity and in this respect is similar to chicken liver pyruvate carboxylase. However, unlike the chicken liver enzyme, the quaternary structure of the enzyme is quite stable in the absence of acetyl CoA, and the major locus of action of this effector appears to lie outside of the stimulation of the biotin carboxylation reaction.

References

Jan 1, 1989·Annual Review of Biochemistry·J R Knowles
Mar 1, 1995·The International Journal of Biochemistry & Cell Biology·P V Attwood

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Citations

Oct 1, 2011·Biochemistry·Tonya N ZeczyckiW Wallace Cleland
Apr 29, 2010·Nucleic Acids Research·Maria ManosasVincent Croquette
Nov 29, 2011·Archives of Biochemistry and Biophysics·Abdussalam Adina-ZadaPaul V Attwood
Jan 28, 2014·Biochemistry·Abdussalam Adina-ZadaPaul V Attwood
Feb 15, 2008·The International Journal of Biochemistry & Cell Biology·Abdussalam Adina-ZadaPaul V Attwood
Jul 31, 2007·The International Journal of Biochemistry & Cell Biology·Sarawut JitrapakdeeJohn C Wallace
May 5, 2007·The International Journal of Biochemistry & Cell Biology·Sarawut JitrapakdeePaul V Attwood
Jul 19, 2002·Yeast
Jul 11, 2008·The Biochemical Journal·Sarawut JitrapakdeePaul V Attwood

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